Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3517 | 3' | -58.5 | NC_001623.1 | + | 90181 | 0.66 | 0.764422 |
Target: 5'- gUCuGCGCuCGCCuCUCCagaagagcguuuGCGGCAGUu -3' miRNA: 3'- -AGuUGCG-GCGGuGAGGgu----------CGUCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 65946 | 0.66 | 0.758719 |
Target: 5'- aCAugGCCGagcuggcgaCGCgagCCGGCAaGCAGCc -3' miRNA: 3'- aGUugCGGCg--------GUGag-GGUCGU-CGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 98706 | 0.66 | 0.758719 |
Target: 5'- uUCAuUGCCGU--CUUCCAGUAGCGuGCu -3' miRNA: 3'- -AGUuGCGGCGguGAGGGUCGUCGU-CG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 18075 | 0.66 | 0.749126 |
Target: 5'- cUAGUGCCGUC-CuccagcaacucgUCCCAGCuGCGGCg -3' miRNA: 3'- aGUUGCGGCGGuG------------AGGGUCGuCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 107383 | 0.66 | 0.73943 |
Target: 5'- gUCGACGCUGgCugUaaCGGCGGCuuguuGCa -3' miRNA: 3'- -AGUUGCGGCgGugAggGUCGUCGu----CG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 118451 | 0.66 | 0.73943 |
Target: 5'- -aAACGCCGUCACgaccgaaaCCAGCuuuuuGcCGGCa -3' miRNA: 3'- agUUGCGGCGGUGag------GGUCGu----C-GUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 89633 | 0.67 | 0.72964 |
Target: 5'- gCAAuCGCCGCCGCggguaacaUCCAGCgaccuGGCcGCa -3' miRNA: 3'- aGUU-GCGGCGGUGa-------GGGUCG-----UCGuCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 87678 | 0.67 | 0.713806 |
Target: 5'- aCGACGuCCGCguUUCCCGccGCguacugagacgcuauGGCAGCg -3' miRNA: 3'- aGUUGC-GGCGguGAGGGU--CG---------------UCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 90759 | 0.67 | 0.699803 |
Target: 5'- aCGACuuGuuGCCgACUCgCAGCAGCAa- -3' miRNA: 3'- aGUUG--CggCGG-UGAGgGUCGUCGUcg -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 5181 | 0.67 | 0.698799 |
Target: 5'- gCAGcCGCCGCCcUUCUUAGCcaggcugAGCGGUa -3' miRNA: 3'- aGUU-GCGGCGGuGAGGGUCG-------UCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 20949 | 0.67 | 0.679612 |
Target: 5'- aCAauGCGCCGaCCguGCUgaaggCCCAgGCAGCGGUu -3' miRNA: 3'- aGU--UGCGGC-GG--UGA-----GGGU-CGUCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 31873 | 0.67 | 0.677584 |
Target: 5'- gUUAACGCCGaCGCUCCUgccuguuugauuacgGGUucuaaaagacgaaacAGCAGCg -3' miRNA: 3'- -AGUUGCGGCgGUGAGGG---------------UCG---------------UCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 5520 | 0.68 | 0.659266 |
Target: 5'- cUCAACGCCacaaaacuuGCCAaaUCUuGUAGCAGCa -3' miRNA: 3'- -AGUUGCGG---------CGGUgaGGGuCGUCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 67394 | 0.68 | 0.645989 |
Target: 5'- aCAACGUCgGCguUUCCUucuugcucaucgauAGCGGCGGCg -3' miRNA: 3'- aGUUGCGG-CGguGAGGG--------------UCGUCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 12638 | 0.68 | 0.638832 |
Target: 5'- -aAACGCCGCCAUUUUuuuaugacgCAGCA-CGGCg -3' miRNA: 3'- agUUGCGGCGGUGAGG---------GUCGUcGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 89810 | 0.68 | 0.618377 |
Target: 5'- aUCGAUGCgGCCAggucgcuggaugUUaCCC-GCGGCGGCg -3' miRNA: 3'- -AGUUGCGgCGGU------------GA-GGGuCGUCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 105676 | 0.69 | 0.597963 |
Target: 5'- -gGGCGUCGCCAUguaCGGCcGCGGCu -3' miRNA: 3'- agUUGCGGCGGUGaggGUCGuCGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 87817 | 0.69 | 0.58779 |
Target: 5'- aCGuCGUCGCCGCg-CCAGCGuccgcGCAGCc -3' miRNA: 3'- aGUuGCGGCGGUGagGGUCGU-----CGUCG- -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 78327 | 0.7 | 0.537538 |
Target: 5'- uUCGcCGCCGCCuuCUCCCAGUccuuugGGCGa- -3' miRNA: 3'- -AGUuGCGGCGGu-GAGGGUCG------UCGUcg -5' |
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3517 | 3' | -58.5 | NC_001623.1 | + | 75681 | 0.7 | 0.527654 |
Target: 5'- gCAAauccuaaCGCUACUCUgGGCGGCGGCg -3' miRNA: 3'- aGUUgcg----GCGGUGAGGgUCGUCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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