Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 354 | 5' | -56.3 | AC_000011.1 | + | 23279 | 0.71 | 0.255088 |
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Target: 5'- gUCGCCUCcGCCucuGCCCccGAAGAGACa -3' miRNA: 3'- -AGCGGGGuCGG---CGGGuuCUUCUUUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 23407 | 0.66 | 0.533273 |
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Target: 5'- cUGCCCCcaCCGCCgaCGAGAAGcAGCa -3' miRNA: 3'- aGCGGGGucGGCGG--GUUCUUCuUUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 23455 | 0.67 | 0.459246 |
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Target: 5'- cCGCCCC-GCCGCCCAGc-------- -3' miRNA: 3'- aGCGGGGuCGGCGGGUUcuucuuugc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 23469 | 0.66 | 0.497754 |
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Target: 5'- -nGCCCCgccaccuccgacgcGGCCGUCCcAGAcaugcaAGAGAUGg -3' miRNA: 3'- agCGGGG--------------UCGGCGGGuUCU------UCUUUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 25489 | 0.66 | 0.544218 |
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Target: 5'- uUC-CCCCAGgaUGCCCc-GAGGAAACa -3' miRNA: 3'- -AGcGGGGUCg-GCGGGuuCUUCUUUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 25950 | 0.68 | 0.409857 |
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Target: 5'- gUUGCCCCcGCagGCCugCAAGcAGGAGACGa -3' miRNA: 3'- -AGCGGGGuCGg-CGG--GUUC-UUCUUUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 26387 | 0.75 | 0.146161 |
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Target: 5'- uUCGCCCUAGCCGCCUccacccaucaucauGAGcaAAGAGAUu -3' miRNA: 3'- -AGCGGGGUCGGCGGG--------------UUC--UUCUUUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 30661 | 0.68 | 0.400381 |
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Target: 5'- -gGCUCCGGCUGCCCGAcAAuGAugGg -3' miRNA: 3'- agCGGGGUCGGCGGGUUcUUcUUugC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 34012 | 0.66 | 0.544218 |
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Target: 5'- aCGCUCCAGCCGaaacucauCgCGGGAAGGAu-- -3' miRNA: 3'- aGCGGGGUCGGC--------GgGUUCUUCUUugc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 34223 | 0.72 | 0.248433 |
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Target: 5'- cCGCCCCGcCCGCCaugCAGcGAAGAGACc -3' miRNA: 3'- aGCGGGGUcGGCGG---GUU-CUUCUUUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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