Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 354 | 5' | -56.3 | AC_000011.1 | + | 20294 | 0.69 | 0.372809 |
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Target: 5'- gCGgCCCAGuuGCgCGAGggGAuggaGAUGg -3' miRNA: 3'- aGCgGGGUCggCGgGUUCuuCU----UUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 8836 | 0.69 | 0.346556 |
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Target: 5'- gCGCgCCGGCCGCggagaccuUCAAGAGGAGGa- -3' miRNA: 3'- aGCGgGGUCGGCG--------GGUUCUUCUUUgc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 19935 | 0.7 | 0.321654 |
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Target: 5'- gCGCCC--GuuGCCCAGGAGcauGGAGCGg -3' miRNA: 3'- aGCGGGguCggCGGGUUCUU---CUUUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 17896 | 0.71 | 0.283175 |
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Target: 5'- cUCGCCCC-GCuUGUCCA-GggGAGGCa -3' miRNA: 3'- -AGCGGGGuCG-GCGGGUuCuuCUUUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 23279 | 0.71 | 0.255088 |
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Target: 5'- gUCGCCUCcGCCucuGCCCccGAAGAGACa -3' miRNA: 3'- -AGCGGGGuCGG---CGGGuuCUUCUUUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 8527 | 0.72 | 0.247119 |
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Target: 5'- cCGCCCCuGCCGCCCGgcgcgcguccucgccGccgcuucuaaccaugGAAGAGGCa -3' miRNA: 3'- aGCGGGGuCGGCGGGU---------------U---------------CUUCUUUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 9327 | 0.76 | 0.12073 |
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Target: 5'- cCGCCaCCAcuGCCGCCUGAGGAGGAAg- -3' miRNA: 3'- aGCGG-GGU--CGGCGGGUUCUUCUUUgc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 23155 | 0.87 | 0.01962 |
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Target: 5'- cCGCCgCuGCCGCCCAAGAAGAAACc -3' miRNA: 3'- aGCGGgGuCGGCGGGUUCUUCUUUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 8463 | 1.1 | 0.000379 |
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Target: 5'- gUCGCCCCAGCCGCCCAAGAAGAAACGg -3' miRNA: 3'- -AGCGGGGUCGGCGGGUUCUUCUUUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 3600 | 0.68 | 0.419471 |
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Target: 5'- aCGCaCUC--CCGCCCAgAGggGAGACGc -3' miRNA: 3'- aGCG-GGGucGGCGGGU-UCuuCUUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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