Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 354 | 5' | -56.3 | AC_000011.1 | + | 13975 | 0.69 | 0.363909 |
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Target: 5'- gUGCCCCcGCgguaccuggCGCCUAcGGAGggGCGg -3' miRNA: 3'- aGCGGGGuCG---------GCGGGUuCUUCuuUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 12940 | 0.81 | 0.058018 |
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Target: 5'- cCGCCCCAGUacgcgcucaGCaCCGAGGAGGAGCGa -3' miRNA: 3'- aGCGGGGUCGg--------CG-GGUUCUUCUUUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 11890 | 0.69 | 0.381855 |
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Target: 5'- -gGCagCCCAGCCGCCgGGccuuGGAGGCGg -3' miRNA: 3'- agCG--GGGUCGGCGGgUUcu--UCUUUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 10922 | 0.67 | 0.449111 |
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Target: 5'- cCGCCgCGGCCGCCgu-GAGcGggGCu -3' miRNA: 3'- aGCGGgGUCGGCGGguuCUU-CuuUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 9327 | 0.76 | 0.12073 |
|
Target: 5'- cCGCCaCCAcuGCCGCCUGAGGAGGAAg- -3' miRNA: 3'- aGCGG-GGU--CGGCGGGUUCUUCUUUgc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 8836 | 0.69 | 0.346556 |
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Target: 5'- gCGCgCCGGCCGCggagaccuUCAAGAGGAGGa- -3' miRNA: 3'- aGCGgGGUCGGCG--------GGUUCUUCUUUgc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 8527 | 0.72 | 0.247119 |
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Target: 5'- cCGCCCCuGCCGCCCGgcgcgcguccucgccGccgcuucuaaccaugGAAGAGGCa -3' miRNA: 3'- aGCGGGGuCGGCGGGU---------------U---------------CUUCUUUGc -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 8463 | 1.1 | 0.000379 |
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Target: 5'- gUCGCCCCAGCCGCCCAAGAAGAAACGg -3' miRNA: 3'- -AGCGGGGUCGGCGGGUUCUUCUUUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 8210 | 0.66 | 0.555236 |
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Target: 5'- -gGCCggAGCUGUCCAGGGuccuGAGACGc -3' miRNA: 3'- agCGGggUCGGCGGGUUCUu---CUUUGC- -5' |
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| 354 | 5' | -56.3 | AC_000011.1 | + | 3600 | 0.68 | 0.419471 |
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Target: 5'- aCGCaCUC--CCGCCCAgAGggGAGACGc -3' miRNA: 3'- aGCG-GGGucGGCGGGU-UCuuCUUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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