Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3587 | 5' | -52.2 | NC_001650.1 | + | 111022 | 0.66 | 0.99083 |
Target: 5'- gGCUGGGcaGCCUggcucUGAGGCUGCUGgCg -3' miRNA: 3'- aUGACUCa-CGGAa----GCUCUGGUGAUgG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 67931 | 0.66 | 0.988124 |
Target: 5'- aUGCUGAG-GCacaucUCGAgGACCAUggGCCu -3' miRNA: 3'- -AUGACUCaCGga---AGCU-CUGGUGa-UGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 146089 | 0.66 | 0.988124 |
Target: 5'- ---gGAG-GCaCUUCGGGGCCGCgUACg -3' miRNA: 3'- augaCUCaCG-GAAGCUCUGGUG-AUGg -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 137215 | 0.66 | 0.988124 |
Target: 5'- gGCUGGGUGgaccCCUUggcgaacacgUGAGGCCccgcggcgcACUGCCa -3' miRNA: 3'- aUGACUCAC----GGAA----------GCUCUGG---------UGAUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 116140 | 0.66 | 0.986558 |
Target: 5'- gGCUGAcauaaacagGUuCCUg-GAGGCCACUGCUg -3' miRNA: 3'- aUGACU---------CAcGGAagCUCUGGUGAUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 114490 | 0.66 | 0.984837 |
Target: 5'- -cCUGAcgauGgcgGCCUUCGGGACCGggaUGCUg -3' miRNA: 3'- auGACU----Ca--CGGAAGCUCUGGUg--AUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 165677 | 0.67 | 0.982954 |
Target: 5'- ---cGGGUGCUUccUCG-GGCCGCUcGCCu -3' miRNA: 3'- augaCUCACGGA--AGCuCUGGUGA-UGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 4464 | 0.67 | 0.982757 |
Target: 5'- gGCUGGGUGgaggguaCCUcgcggucaUCGcauGACCAUUACCu -3' miRNA: 3'- aUGACUCAC-------GGA--------AGCu--CUGGUGAUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 171171 | 0.67 | 0.982757 |
Target: 5'- gGCUGGGUGgaggguaCCUcgcggucaUCGcauGACCAUUACCu -3' miRNA: 3'- aUGACUCAC-------GGA--------AGCu--CUGGUGAUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 114174 | 0.67 | 0.9809 |
Target: 5'- -uCUGuGUGgcugcccgucaCCUUCGAGGCUGuCUACCu -3' miRNA: 3'- auGACuCAC-----------GGAAGCUCUGGU-GAUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 68205 | 0.67 | 0.9809 |
Target: 5'- ---gGAGUGCCUguaCGAGugCGCcuCCu -3' miRNA: 3'- augaCUCACGGAa--GCUCugGUGauGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 156543 | 0.67 | 0.978666 |
Target: 5'- gGCUGAGgcacccccUGCCccccgUCGAGGCC-C-ACCg -3' miRNA: 3'- aUGACUC--------ACGGa----AGCUCUGGuGaUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 76844 | 0.67 | 0.976244 |
Target: 5'- gGCcGAGaaggcgcgcGCCUUCGAGACCuucauCUACg -3' miRNA: 3'- aUGaCUCa--------CGGAAGCUCUGGu----GAUGg -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 156907 | 0.68 | 0.97196 |
Target: 5'- aUGCUGGGaccgGCCUUUGAgcuggcccagcuccaGAUCACcGCCg -3' miRNA: 3'- -AUGACUCa---CGGAAGCU---------------CUGGUGaUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 126072 | 0.68 | 0.964531 |
Target: 5'- gUGCUGGGUGCgCUguuggCGAgcGugCACcGCCu -3' miRNA: 3'- -AUGACUCACG-GAa----GCU--CugGUGaUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 126008 | 0.68 | 0.957365 |
Target: 5'- cGCUGGGauugauccGCCUUCGAcGCCGC-GCCc -3' miRNA: 3'- aUGACUCa-------CGGAAGCUcUGGUGaUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 129000 | 0.68 | 0.957365 |
Target: 5'- aGgUGAGgcagGCCUUCGAGGgCGCggGCg -3' miRNA: 3'- aUgACUCa---CGGAAGCUCUgGUGa-UGg -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 83373 | 0.69 | 0.944861 |
Target: 5'- cACcGAGUGgCgggcCGAGGCC-CUGCCg -3' miRNA: 3'- aUGaCUCACgGaa--GCUCUGGuGAUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 154995 | 0.69 | 0.94021 |
Target: 5'- -cCUGAGcccgGCCacccUUCGGGgcacgugcuGCCACUGCCa -3' miRNA: 3'- auGACUCa---CGG----AAGCUC---------UGGUGAUGG- -5' |
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3587 | 5' | -52.2 | NC_001650.1 | + | 60046 | 0.69 | 0.935313 |
Target: 5'- -cCUGGGcUGCCUgccCGuGACCAUgGCCg -3' miRNA: 3'- auGACUC-ACGGAa--GCuCUGGUGaUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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