Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3587 | 5' | -52.2 | NC_001650.1 | + | 9069 | 0.69 | 0.935313 |
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Target: 5'- -uCUuGGUGCaCUUUGGGACCACUAgUg -3' miRNA: 3'- auGAcUCACG-GAAGCUCUGGUGAUgG- -5' |
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| 3587 | 5' | -52.2 | NC_001650.1 | + | 175776 | 0.69 | 0.935313 |
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Target: 5'- -uCUuGGUGCaCUUUGGGACCACUAgUg -3' miRNA: 3'- auGAcUCACG-GAAGCUCUGGUGAUgG- -5' |
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| 3587 | 5' | -52.2 | NC_001650.1 | + | 115873 | 0.69 | 0.935313 |
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Target: 5'- gGCUGAGUGCCguacacCGGGcCCACg--- -3' miRNA: 3'- aUGACUCACGGaa----GCUCuGGUGaugg -5' |
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| 3587 | 5' | -52.2 | NC_001650.1 | + | 44112 | 0.7 | 0.919136 |
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Target: 5'- ---aGAGUGCCUg-GAGAucccuCCAUUACCa -3' miRNA: 3'- augaCUCACGGAagCUCU-----GGUGAUGG- -5' |
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| 3587 | 5' | -52.2 | NC_001650.1 | + | 19349 | 0.7 | 0.919136 |
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Target: 5'- -gUUGAGUGCCaguuUUCuGGGGCCACaGCUg -3' miRNA: 3'- auGACUCACGG----AAG-CUCUGGUGaUGG- -5' |
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| 3587 | 5' | -52.2 | NC_001650.1 | + | 129997 | 0.71 | 0.894118 |
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Target: 5'- cUGCUGAGggcgGCgCgggaCGAGGCCGCggGCCu -3' miRNA: 3'- -AUGACUCa---CG-Gaa--GCUCUGGUGa-UGG- -5' |
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| 3587 | 5' | -52.2 | NC_001650.1 | + | 77769 | 0.75 | 0.710107 |
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Target: 5'- -uUUGAGgaaUGUCUggaguuuuucUCGAGGCCGCUGCCg -3' miRNA: 3'- auGACUC---ACGGA----------AGCUCUGGUGAUGG- -5' |
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| 3587 | 5' | -52.2 | NC_001650.1 | + | 52977 | 0.77 | 0.585733 |
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Target: 5'- aUACUcggccGGUGCCgcgUCGAGGCgCGCUGCCu -3' miRNA: 3'- -AUGAc----UCACGGa--AGCUCUG-GUGAUGG- -5' |
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| 3587 | 5' | -52.2 | NC_001650.1 | + | 175950 | 1.11 | 0.005349 |
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Target: 5'- cUACUGAGUGCCUUCGAGACCACUACCa -3' miRNA: 3'- -AUGACUCACGGAAGCUCUGGUGAUGG- -5' |
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| 3587 | 5' | -52.2 | NC_001650.1 | + | 9243 | 1.11 | 0.005349 |
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Target: 5'- cUACUGAGUGCCUUCGAGACCACUACCa -3' miRNA: 3'- -AUGACUCACGGAAGCUCUGGUGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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