Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3588 | 3' | -56.9 | NC_001650.1 | + | 158971 | 0.66 | 0.919988 |
Target: 5'- uGCUGGuGGUCaucuuaaggaauGGGUCcggGGAUgAGGa -3' miRNA: 3'- gCGACC-CCAG------------UCCAGagaCCUAgUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 12783 | 0.66 | 0.919988 |
Target: 5'- gGCUaGGGUUAGG-CUa-GGGUUAGGg -3' miRNA: 3'- gCGAcCCCAGUCCaGAgaCCUAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 147617 | 0.66 | 0.919988 |
Target: 5'- cCGCUacaGGGGaaccgcuuUUGGGUCgCUGGcUCGGGg -3' miRNA: 3'- -GCGA---CCCC--------AGUCCAGaGACCuAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 69413 | 0.66 | 0.919988 |
Target: 5'- aGCUGGGGguugaugaGGGUCa--GGcUCAGGg -3' miRNA: 3'- gCGACCCCag------UCCAGagaCCuAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 155454 | 0.66 | 0.919988 |
Target: 5'- uGCUGGGcGUaCGGGaCgcacCUGGGggUCAGGu -3' miRNA: 3'- gCGACCC-CA-GUCCaGa---GACCU--AGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 57550 | 0.66 | 0.919438 |
Target: 5'- gGCUGGGGcugggccUguGGUUgcagcugcggCUGGggCAGGg -3' miRNA: 3'- gCGACCCC-------AguCCAGa---------GACCuaGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 151519 | 0.66 | 0.90857 |
Target: 5'- gGCUccGGuUCAGG-CUCUGGcUCAGGu -3' miRNA: 3'- gCGAccCC-AGUCCaGAGACCuAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 151477 | 0.66 | 0.90857 |
Target: 5'- gGCUccGGuUCAGG-CUCUGGcUCAGGu -3' miRNA: 3'- gCGAccCC-AGUCCaGAGACCuAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 151435 | 0.66 | 0.90857 |
Target: 5'- gGCUccGGuUCAGG-CUCUGGcUCAGGu -3' miRNA: 3'- gCGAccCC-AGUCCaGAGACCuAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 151393 | 0.66 | 0.90857 |
Target: 5'- gGCUccGGuUCAGG-CUCUGGuUCAGGu -3' miRNA: 3'- gCGAccCC-AGUCCaGAGACCuAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 131445 | 0.66 | 0.902524 |
Target: 5'- gCGUUGGGcaccaGGGUCUCggGGAcCGGGu -3' miRNA: 3'- -GCGACCCcag--UCCAGAGa-CCUaGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 52408 | 0.67 | 0.89239 |
Target: 5'- aGCgUGGGaaaauauuucaaagaGUgGgGGUCUCUGGcgCAGGg -3' miRNA: 3'- gCG-ACCC---------------CAgU-CCAGAGACCuaGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 171358 | 0.67 | 0.889769 |
Target: 5'- ---aGGGGUCuuuguccgggGGGUCUUUGGGggucCGGGg -3' miRNA: 3'- gcgaCCCCAG----------UCCAGAGACCUa---GUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 4651 | 0.67 | 0.889769 |
Target: 5'- ---aGGGGUCuuuguccgggGGGUCUUUGGGggucCGGGg -3' miRNA: 3'- gcgaCCCCAG----------UCCAGAGACCUa---GUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 90124 | 0.67 | 0.889769 |
Target: 5'- cCGC-GGGGUCugGGGcCUC-GG-UCAGGg -3' miRNA: 3'- -GCGaCCCCAG--UCCaGAGaCCuAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 145498 | 0.67 | 0.883067 |
Target: 5'- gGCcgGGGGcUCcuGGUCagCUGGAgcuUCAGGg -3' miRNA: 3'- gCGa-CCCC-AGu-CCAGa-GACCU---AGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 88524 | 0.67 | 0.869031 |
Target: 5'- aGCgGGGGcgCcGGUCUCUGGAaaAGa -3' miRNA: 3'- gCGaCCCCa-GuCCAGAGACCUagUCc -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 1953 | 0.67 | 0.869031 |
Target: 5'- gGCcaaUGGGGagAGGgugggUUGGGUCAGGg -3' miRNA: 3'- gCG---ACCCCagUCCaga--GACCUAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 168660 | 0.67 | 0.869031 |
Target: 5'- gGCcaaUGGGGagAGGgugggUUGGGUCAGGg -3' miRNA: 3'- gCG---ACCCCagUCCaga--GACCUAGUCC- -5' |
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3588 | 3' | -56.9 | NC_001650.1 | + | 139062 | 0.68 | 0.846474 |
Target: 5'- aGCUGGGGcaaaCAuGGUCuUCUGGGgauGGGa -3' miRNA: 3'- gCGACCCCa---GU-CCAG-AGACCUag-UCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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