Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3589 | 5' | -47.7 | NC_001650.1 | + | 71382 | 0.66 | 0.999879 |
Target: 5'- cCCUGCgccGGCGcgcUCCA--GGCCGCc -3' miRNA: 3'- -GGACGa--UCGUauuAGGUuaUCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 88259 | 0.66 | 0.999843 |
Target: 5'- uCCUGUgggAGCGggagcUCCGG-GGCCACa -3' miRNA: 3'- -GGACGa--UCGUauu--AGGUUaUCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 43910 | 0.66 | 0.99974 |
Target: 5'- aUCUGgUGGCAgac-CCuGUGGCCGCc -3' miRNA: 3'- -GGACgAUCGUauuaGGuUAUCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 65206 | 0.66 | 0.99974 |
Target: 5'- aCgUGCUGGCccgcguccUCCAGgccGGCCACg -3' miRNA: 3'- -GgACGAUCGuauu----AGGUUa--UCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 180145 | 0.66 | 0.9997 |
Target: 5'- gCCUGaggcacuaucaaaGGCAUAAUCCAAaGGCUAUUu -3' miRNA: 3'- -GGACga-----------UCGUAUUAGGUUaUCGGUGA- -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 13438 | 0.66 | 0.9997 |
Target: 5'- gCCUGaggcacuaucaaaGGCAUAAUCCAAaGGCUAUUu -3' miRNA: 3'- -GGACga-----------UCGUAUUAGGUUaUCGGUGA- -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 178752 | 0.66 | 0.999654 |
Target: 5'- -aUGCUAGCAggagcagUAuagauccaaacuaAUCCAGUAGCCuCUu -3' miRNA: 3'- ggACGAUCGU-------AU-------------UAGGUUAUCGGuGA- -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 12045 | 0.66 | 0.999654 |
Target: 5'- -aUGCUAGCAggagcagUAuagauccaaacuaAUCCAGUAGCCuCUu -3' miRNA: 3'- ggACGAUCGU-------AU-------------UAGGUUAUCGGuGA- -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 138700 | 0.67 | 0.999583 |
Target: 5'- gUCUGCcAGCAUGGccccugucUCCAgcAUGGCCcCUg -3' miRNA: 3'- -GGACGaUCGUAUU--------AGGU--UAUCGGuGA- -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 60975 | 0.67 | 0.999478 |
Target: 5'- cUCUGCaUGGCGcuGUCgGucAUGGCCGCg -3' miRNA: 3'- -GGACG-AUCGUauUAGgU--UAUCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 62176 | 0.67 | 0.999478 |
Target: 5'- --aGCUGGCAaagUAuUCCAGcgugGGCCACc -3' miRNA: 3'- ggaCGAUCGU---AUuAGGUUa---UCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 156478 | 0.67 | 0.999478 |
Target: 5'- cCCUGgUGGCccgcgggcugGUGA-CCGcgGGCCACg -3' miRNA: 3'- -GGACgAUCG----------UAUUaGGUuaUCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 164370 | 0.67 | 0.99935 |
Target: 5'- uCCUGCUgcgAGUGUGGUCCGc--GCUGCa -3' miRNA: 3'- -GGACGA---UCGUAUUAGGUuauCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 68310 | 0.67 | 0.99901 |
Target: 5'- cCCUGCaGGUccaggGUGGaguUCCAGUuGGCCACg -3' miRNA: 3'- -GGACGaUCG-----UAUU---AGGUUA-UCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 175093 | 0.67 | 0.99901 |
Target: 5'- cCCUGCcccuCAUAG-CCAuUGGCCACa -3' miRNA: 3'- -GGACGauc-GUAUUaGGUuAUCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 8386 | 0.67 | 0.99901 |
Target: 5'- cCCUGCcccuCAUAG-CCAuUGGCCACa -3' miRNA: 3'- -GGACGauc-GUAUUaGGUuAUCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 51517 | 0.68 | 0.99879 |
Target: 5'- gCCgaGCUGGCccag-CCAcUGGCCACg -3' miRNA: 3'- -GGa-CGAUCGuauuaGGUuAUCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 29366 | 0.68 | 0.998223 |
Target: 5'- aCCUGCUcaggcGCAgcGUCCAGggGGCgGCg -3' miRNA: 3'- -GGACGAu----CGUauUAGGUUa-UCGgUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 99075 | 0.68 | 0.997865 |
Target: 5'- gCCU-CUGGCA--GUCCAcgcAGCCACa -3' miRNA: 3'- -GGAcGAUCGUauUAGGUua-UCGGUGa -5' |
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3589 | 5' | -47.7 | NC_001650.1 | + | 142339 | 0.68 | 0.997865 |
Target: 5'- cCCgGCUGGaaggaGUGcUCCAggAGCCGCg -3' miRNA: 3'- -GGaCGAUCg----UAUuAGGUuaUCGGUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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