Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 392 | 3' | -61.1 | AC_000011.1 | + | 21672 | 0.66 | 0.318292 |
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Target: 5'- uUCGCUCCCAcCGCgCGCGCaUCGAgAa- -3' miRNA: 3'- -GGUGGGGGUcGCG-GCGCG-AGCUgUac -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 23303 | 0.66 | 0.333993 |
|
Target: 5'- gCGCUCCCGGCG--GCGCUCuGAC-UGa -3' miRNA: 3'- gGUGGGGGUCGCggCGCGAG-CUGuAC- -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 23459 | 0.66 | 0.310652 |
|
Target: 5'- cCCGCCgCCCAGCcCCGCcacCUcCGACGc- -3' miRNA: 3'- -GGUGG-GGGUCGcGGCGc--GA-GCUGUac -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 25694 | 0.66 | 0.342054 |
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Target: 5'- gCCGCCgCCAGacCGUCGUcCUCGGCGg- -3' miRNA: 3'- -GGUGGgGGUC--GCGGCGcGAGCUGUac -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 26496 | 0.67 | 0.281492 |
|
Target: 5'- uCCACCCgcaugaauuggcUCAGCGCCGgGCccgCGAUg-- -3' miRNA: 3'- -GGUGGG------------GGUCGCGGCgCGa--GCUGuac -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 30912 | 0.71 | 0.142255 |
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Target: 5'- aCCACCCCCAGUaCCGCGaccagCGAgGg- -3' miRNA: 3'- -GGUGGGGGUCGcGGCGCga---GCUgUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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