Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
392 | 3' | -61.1 | AC_000011.1 | + | 16557 | 0.67 | 0.295792 |
Target: 5'- gCCGaCCgCGGCGCCGCGC-CGGgGg- -3' miRNA: 3'- -GGUgGGgGUCGCGGCGCGaGCUgUac -5' |
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392 | 3' | -61.1 | AC_000011.1 | + | 23459 | 0.66 | 0.310652 |
Target: 5'- cCCGCCgCCCAGCcCCGCcacCUcCGACGc- -3' miRNA: 3'- -GGUGG-GGGUCGcGGCGc--GA-GCUGUac -5' |
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392 | 3' | -61.1 | AC_000011.1 | + | 21672 | 0.66 | 0.318292 |
Target: 5'- uUCGCUCCCAcCGCgCGCGCaUCGAgAa- -3' miRNA: 3'- -GGUGGGGGUcGCG-GCGCG-AGCUgUac -5' |
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392 | 3' | -61.1 | AC_000011.1 | + | 8197 | 0.66 | 0.318292 |
Target: 5'- cCUGCCUCCcGCGCgG-GCUCGucaGCAUGa -3' miRNA: 3'- -GGUGGGGGuCGCGgCgCGAGC---UGUAC- -5' |
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392 | 3' | -61.1 | AC_000011.1 | + | 23303 | 0.66 | 0.333993 |
Target: 5'- gCGCUCCCGGCG--GCGCUCuGAC-UGa -3' miRNA: 3'- gGUGGGGGUCGCggCGCGAG-CUGuAC- -5' |
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392 | 3' | -61.1 | AC_000011.1 | + | 25694 | 0.66 | 0.342054 |
Target: 5'- gCCGCCgCCAGacCGUCGUcCUCGGCGg- -3' miRNA: 3'- -GGUGGgGGUC--GCGGCGcGAGCUGUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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