Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 392 | 3' | -61.1 | AC_000011.1 | + | 9919 | 0.71 | 0.150222 |
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Target: 5'- uCCAgCCCCAGCGCgGCuucggGCUCacgcGCAUGc -3' miRNA: 3'- -GGUgGGGGUCGCGgCG-----CGAGc---UGUAC- -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 30912 | 0.71 | 0.142255 |
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Target: 5'- aCCACCCCCAGUaCCGCGaccagCGAgGg- -3' miRNA: 3'- -GGUGGGGGUCGcGGCGCga---GCUgUac -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 3686 | 0.73 | 0.099266 |
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Target: 5'- uCUGCCgCCAGCGCCGUGCgCGGaAUGg -3' miRNA: 3'- -GGUGGgGGUCGCGGCGCGaGCUgUAC- -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 15685 | 0.73 | 0.099266 |
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Target: 5'- aCACCCCCGcCGCCGCGCcCGucuccacCGUGg -3' miRNA: 3'- gGUGGGGGUcGCGGCGCGaGCu------GUAC- -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 16618 | 0.78 | 0.041005 |
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Target: 5'- -aACCCCCggcgcGGCGCCGCGgUCGGCAg- -3' miRNA: 3'- ggUGGGGG-----UCGCGGCGCgAGCUGUac -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 13026 | 1.09 | 0.000154 |
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Target: 5'- gCCACCCCCAGCGCCGCGCUCGACAUGa -3' miRNA: 3'- -GGUGGGGGUCGCGGCGCGAGCUGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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