Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 392 | 3' | -61.1 | AC_000011.1 | + | 9365 | 0.67 | 0.261078 |
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Target: 5'- gUCugCCCguGCGCCGCcggCGACGc- -3' miRNA: 3'- -GGugGGGguCGCGGCGcgaGCUGUac -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 21316 | 0.68 | 0.254546 |
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Target: 5'- cUCGCCCCCGGUcucgcggccgGCCGUGUU-GACgAUGg -3' miRNA: 3'- -GGUGGGGGUCG----------CGGCGCGAgCUG-UAC- -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 16863 | 0.68 | 0.235754 |
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Target: 5'- gCACCUCCauggugcuGGUGCUGgGCUUGAuCAUGg -3' miRNA: 3'- gGUGGGGG--------UCGCGGCgCGAGCU-GUAC- -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 9919 | 0.71 | 0.150222 |
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Target: 5'- uCCAgCCCCAGCGCgGCuucggGCUCacgcGCAUGc -3' miRNA: 3'- -GGUgGGGGUCGCGgCG-----CGAGc---UGUAC- -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 16618 | 0.78 | 0.041005 |
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Target: 5'- -aACCCCCggcgcGGCGCCGCGgUCGGCAg- -3' miRNA: 3'- ggUGGGGG-----UCGCGGCGCgAGCUGUac -5' |
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| 392 | 3' | -61.1 | AC_000011.1 | + | 8197 | 0.66 | 0.318292 |
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Target: 5'- cCUGCCUCCcGCGCgG-GCUCGucaGCAUGa -3' miRNA: 3'- -GGUGGGGGuCGCGgCgCGAGC---UGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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