Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 408 | 3' | -55.6 | AC_000011.1 | + | 23644 | 0.66 | 0.558844 |
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Target: 5'- aGGCUGGgcucgagcaUGACGgCGacuaccuCCACCUGAGCg- -3' miRNA: 3'- -CCGGCC---------AUUGCgGU-------GGUGGAUUCGag -5' |
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| 408 | 3' | -55.6 | AC_000011.1 | + | 5231 | 0.66 | 0.559959 |
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Target: 5'- cGCCGGgcugGGCGCUugCgagggugcGCUUcAGGCUCa -3' miRNA: 3'- cCGGCCa---UUGCGGugG--------UGGA-UUCGAG- -5' |
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| 408 | 3' | -55.6 | AC_000011.1 | + | 15750 | 0.66 | 0.571137 |
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Target: 5'- cGCCGGUAcgcccGCGCCAagaGCCggcGGCg- -3' miRNA: 3'- cCGGCCAU-----UGCGGUgg-UGGau-UCGag -5' |
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| 408 | 3' | -55.6 | AC_000011.1 | + | 31693 | 0.66 | 0.571137 |
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Target: 5'- aGGCaGGUGaacuucugGCGCUGCUGCagGAGCUCg -3' miRNA: 3'- -CCGgCCAU--------UGCGGUGGUGgaUUCGAG- -5' |
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| 408 | 3' | -55.6 | AC_000011.1 | + | 17219 | 0.66 | 0.590258 |
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Target: 5'- aGGCUGGcGGggaacgggaugcguCGCCACCACCaccGGCg- -3' miRNA: 3'- -CCGGCCaUU--------------GCGGUGGUGGau-UCGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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