Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 408 | 3' | -55.6 | AC_000011.1 | + | 26806 | 0.69 | 0.39312 |
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Target: 5'- gGGgCGGUGacgacacagggugGCGCCGCCcgccagcuggacACCUGAGUUa -3' miRNA: 3'- -CCgGCCAU-------------UGCGGUGG------------UGGAUUCGAg -5' |
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| 408 | 3' | -55.6 | AC_000011.1 | + | 31578 | 0.67 | 0.473382 |
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Target: 5'- -aCUGGUggAugGCCACCGcauCCUGcAGCUCc -3' miRNA: 3'- ccGGCCA--UugCGGUGGU---GGAU-UCGAG- -5' |
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| 408 | 3' | -55.6 | AC_000011.1 | + | 31693 | 0.66 | 0.571137 |
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Target: 5'- aGGCaGGUGaacuucugGCGCUGCUGCagGAGCUCg -3' miRNA: 3'- -CCGgCCAU--------UGCGGUGGUGgaUUCGAG- -5' |
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| 408 | 3' | -55.6 | AC_000011.1 | + | 33787 | 0.68 | 0.467148 |
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Target: 5'- cGGCCGGUGgugucgcaucagGCcccgcagcagucgcuGCCGCCGCC---GCUCc -3' miRNA: 3'- -CCGGCCAU------------UG---------------CGGUGGUGGauuCGAG- -5' |
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| 408 | 3' | -55.6 | AC_000011.1 | + | 35287 | 0.69 | 0.413081 |
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Target: 5'- uGCCGGUGgaggGCGCuCugCGC--GAGCUCu -3' miRNA: 3'- cCGGCCAU----UGCG-GugGUGgaUUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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