Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
408 | 3' | -55.6 | AC_000011.1 | + | 22416 | 0.72 | 0.243227 |
Target: 5'- cGCCGGguGCGCCACCaACC--AGUUCu -3' miRNA: 3'- cCGGCCauUGCGGUGG-UGGauUCGAG- -5' |
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408 | 3' | -55.6 | AC_000011.1 | + | 8908 | 0.73 | 0.212457 |
Target: 5'- cGCCGGc-GCGCaugACCACCUGGGCg- -3' miRNA: 3'- cCGGCCauUGCGg--UGGUGGAUUCGag -5' |
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408 | 3' | -55.6 | AC_000011.1 | + | 9248 | 0.78 | 0.104529 |
Target: 5'- gGGCCuucgagcgcgaGGUGcGCGCCACCAUCgccGAGCUCa -3' miRNA: 3'- -CCGG-----------CCAU-UGCGGUGGUGGa--UUCGAG- -5' |
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408 | 3' | -55.6 | AC_000011.1 | + | 15831 | 0.82 | 0.051274 |
Target: 5'- cGCCGGgcgauGCGCCGCCGCC--GGCUCu -3' miRNA: 3'- cCGGCCau---UGCGGUGGUGGauUCGAG- -5' |
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408 | 3' | -55.6 | AC_000011.1 | + | 21073 | 1.12 | 0.000323 |
Target: 5'- cGGCCGGUAACGCCACCACCUAAGCUCu -3' miRNA: 3'- -CCGGCCAUUGCGGUGGUGGAUUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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