Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
408 | 5' | -62.7 | AC_000011.1 | + | 21108 | 1.1 | 0.000093 |
Target: 5'- uGCAAGCCAUGGCCGCGGGCUCCGGCGa -3' miRNA: 3'- -CGUUCGGUACCGGCGCCCGAGGCCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 21146 | 0.66 | 0.26698 |
Target: 5'- -aGGGCCAUcauccgcgaccugGGCUGCGGGCccuacuUCCugGGCa -3' miRNA: 3'- cgUUCGGUA-------------CCGGCGCCCG------AGG--CCGc -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 21359 | 0.66 | 0.254499 |
Target: 5'- aGCAGGUguUcGagCGCGGGUUCCaGGCGa -3' miRNA: 3'- -CGUUCGguAcCg-GCGCCCGAGG-CCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 23060 | 0.7 | 0.138686 |
Target: 5'- -gGAGCUGgcGGCCGUGGGCUucagcuaccccCCGGUGc -3' miRNA: 3'- cgUUCGGUa-CCGGCGCCCGA-----------GGCCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 23951 | 0.66 | 0.248124 |
Target: 5'- aGguGGCCAgGGCCuCGGGCaCC-GCGa -3' miRNA: 3'- -CguUCGGUaCCGGcGCCCGaGGcCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 24314 | 0.69 | 0.158824 |
Target: 5'- gGCAAGcCCGUGGUCaGCGacgagcagcuGGC-CCGGUGg -3' miRNA: 3'- -CGUUC-GGUACCGG-CGC----------CCGaGGCCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 35746 | 0.68 | 0.172141 |
Target: 5'- cCAGGCa--GGCCaCGGGguCUCCGGCGc -3' miRNA: 3'- cGUUCGguaCCGGcGCCC--GAGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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