Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
408 | 5' | -62.7 | AC_000011.1 | + | 18146 | 0.68 | 0.176796 |
Target: 5'- gGCGGGCgGUGcccccgggucGCgCGCGGGCcacggccaCCGGCGg -3' miRNA: 3'- -CGUUCGgUAC----------CG-GCGCCCGa-------GGCCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 23060 | 0.7 | 0.138686 |
Target: 5'- -gGAGCUGgcGGCCGUGGGCUucagcuaccccCCGGUGc -3' miRNA: 3'- cgUUCGGUa-CCGGCGCCCGA-----------GGCCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 15813 | 0.7 | 0.131307 |
Target: 5'- cGCcGGCUcuUGG-CGCGGGCguaCCGGCGc -3' miRNA: 3'- -CGuUCGGu-ACCgGCGCCCGa--GGCCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 15864 | 0.7 | 0.124292 |
Target: 5'- cGCGccgcGCgCAUGGCgGgGGuGCUCCGGUGc -3' miRNA: 3'- -CGUu---CG-GUACCGgCgCC-CGAGGCCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 18057 | 0.73 | 0.081827 |
Target: 5'- gGCAAGuCCAgGGUCGCGGGCUuuUCGGg- -3' miRNA: 3'- -CGUUC-GGUaCCGGCGCCCGA--GGCCgc -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 15976 | 0.73 | 0.075182 |
Target: 5'- ---cGCCGUGGCCGCgcgucuccGGGUccugCCGGCGc -3' miRNA: 3'- cguuCGGUACCGGCG--------CCCGa---GGCCGC- -5' |
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408 | 5' | -62.7 | AC_000011.1 | + | 3230 | 0.66 | 0.274429 |
Target: 5'- uCAGGCucacucuggaCAUGGCauCGGGCUCCaGCa -3' miRNA: 3'- cGUUCG----------GUACCGgcGCCCGAGGcCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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