Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 408 | 5' | -62.7 | AC_000011.1 | + | 16997 | 0.66 | 0.248124 |
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Target: 5'- -gAAGCgCGU-GCCGCGGuaGC-CCGGCGu -3' miRNA: 3'- cgUUCG-GUAcCGGCGCC--CGaGGCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 23951 | 0.66 | 0.248124 |
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Target: 5'- aGguGGCCAgGGCCuCGGGCaCC-GCGa -3' miRNA: 3'- -CguUCGGUaCCGGcGCCCGaGGcCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 21359 | 0.66 | 0.254499 |
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Target: 5'- aGCAGGUguUcGagCGCGGGUUCCaGGCGa -3' miRNA: 3'- -CGUUCGguAcCg-GCGCCCGAGG-CCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 15769 | 0.66 | 0.261007 |
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Target: 5'- -aGAGCC--GGCgGCGGcGCaucgcCCGGCGg -3' miRNA: 3'- cgUUCGGuaCCGgCGCC-CGa----GGCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 12656 | 0.66 | 0.261007 |
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Target: 5'- uGCAGGCCccGGUCG-GGGa-CCGcGCGa -3' miRNA: 3'- -CGUUCGGuaCCGGCgCCCgaGGC-CGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 21146 | 0.66 | 0.26698 |
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Target: 5'- -aGGGCCAUcauccgcgaccugGGCUGCGGGCccuacuUCCugGGCa -3' miRNA: 3'- cgUUCGGUA-------------CCGGCGCCCG------AGG--CCGc -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 3230 | 0.66 | 0.274429 |
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Target: 5'- uCAGGCucacucuggaCAUGGCauCGGGCUCCaGCa -3' miRNA: 3'- cGUUCG----------GUACCGgcGCCCGAGGcCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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