Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 408 | 5' | -62.7 | AC_000011.1 | + | 15864 | 0.7 | 0.124292 |
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Target: 5'- cGCGccgcGCgCAUGGCgGgGGuGCUCCGGUGc -3' miRNA: 3'- -CGUu---CG-GUACCGgCgCC-CGAGGCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 3704 | 0.71 | 0.117626 |
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Target: 5'- cGCGgaauGGCCAUGGgCGCcGGCUacuaCGGCa -3' miRNA: 3'- -CGU----UCGGUACCgGCGcCCGAg---GCCGc -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 18069 | 0.71 | 0.114419 |
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Target: 5'- cGguGGCCGUGGcCCGCGcGCgacCCGGgGg -3' miRNA: 3'- -CguUCGGUACC-GGCGCcCGa--GGCCgC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 11494 | 0.71 | 0.114103 |
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Target: 5'- cCGAGCCcgagGGCCGCuGGCUCCuggaccuGGUGa -3' miRNA: 3'- cGUUCGGua--CCGGCGcCCGAGG-------CCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 18057 | 0.73 | 0.081827 |
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Target: 5'- gGCAAGuCCAgGGUCGCGGGCUuuUCGGg- -3' miRNA: 3'- -CGUUC-GGUaCCGGCGCCCGA--GGCCgc -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 15976 | 0.73 | 0.075182 |
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Target: 5'- ---cGCCGUGGCCGCgcgucuccGGGUccugCCGGCGc -3' miRNA: 3'- cguuCGGUACCGGCG--------CCCGa---GGCCGC- -5' |
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| 408 | 5' | -62.7 | AC_000011.1 | + | 21108 | 1.1 | 0.000093 |
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Target: 5'- uGCAAGCCAUGGCCGCGGGCUCCGGCGa -3' miRNA: 3'- -CGUUCGGUACCGGCGCCCGAGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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