Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 411 | 3' | -60.8 | AC_000011.1 | + | 21464 | 0.68 | 0.240647 |
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Target: 5'- aGCGguCCuCGGUGgccagggCGcUGCgGCGCAGCAg -3' miRNA: 3'- -CGCguGG-GCCGCa------GC-ACG-UGCGUCGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 22189 | 0.67 | 0.259907 |
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Target: 5'- aCGCACCCGGgcuUGUgGuUGCAauCGCAGUg -3' miRNA: 3'- cGCGUGGGCC---GCAgC-ACGU--GCGUCGu -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 22368 | 1.1 | 0.000149 |
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Target: 5'- gGCGCACCCGGCGUCGUGCACGCAGCAg -3' miRNA: 3'- -CGCGUGGGCCGCAGCACGUGCGUCGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 22599 | 0.66 | 0.324423 |
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Target: 5'- cGCGCACCCGGUGcacucccaguucUUGUGggcgaucugggaaUGCGCGuGCAc -3' miRNA: 3'- -CGCGUGGGCCGC------------AGCAC-------------GUGCGU-CGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 24043 | 0.67 | 0.259245 |
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Target: 5'- gGCGCcgggACCCagguugaaaagGGCGUCG-GCgcgggugcgguugGCGCGGCAg -3' miRNA: 3'- -CGCG----UGGG-----------CCGCAGCaCG-------------UGCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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