Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 411 | 3' | -60.8 | AC_000011.1 | + | 22599 | 0.66 | 0.324423 |
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Target: 5'- cGCGCACCCGGUGcacucccaguucUUGUGggcgaucugggaaUGCGCGuGCAc -3' miRNA: 3'- -CGCGUGGGCCGC------------AGCAC-------------GUGCGU-CGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 15909 | 0.66 | 0.325212 |
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Target: 5'- -aGCaugGCCCuGCGUccCGUGCgccuggcccuGCGCAGCAa -3' miRNA: 3'- cgCG---UGGGcCGCA--GCACG----------UGCGUCGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 16995 | 0.66 | 0.333177 |
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Target: 5'- aGCGCgugccgcgguaGCCCGGCGUgGggaugauggaaGgAUGCAGCGc -3' miRNA: 3'- -CGCG-----------UGGGCCGCAgCa----------CgUGCGUCGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 15927 | 0.66 | 0.333177 |
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Target: 5'- cGCGCgGCCaCGGCGgCG-GCA-GCGGCc -3' miRNA: 3'- -CGCG-UGG-GCCGCaGCaCGUgCGUCGu -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 8469 | 0.66 | 0.341285 |
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Target: 5'- cGCGCGCCgGGCGgcaggggCG-GCuCGgGGCc -3' miRNA: 3'- -CGCGUGGgCCGCa------GCaCGuGCgUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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