Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 411 | 3' | -60.8 | AC_000011.1 | + | 16259 | 0.72 | 0.129686 |
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Target: 5'- uGCGCGaguucgcccCCCGGCGgcgCGUGCA-GUGGCGc -3' miRNA: 3'- -CGCGU---------GGGCCGCa--GCACGUgCGUCGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 16058 | 0.72 | 0.126136 |
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Target: 5'- cGCGCACaccgguggCGGCGUCGcGCACcCAGUAc -3' miRNA: 3'- -CGCGUGg-------GCCGCAGCaCGUGcGUCGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 15781 | 0.75 | 0.069059 |
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Target: 5'- gGCGCaucGCCCGGCGgcacCGgagcacccccgccaUGCGCGCGGCGc -3' miRNA: 3'- -CGCG---UGGGCCGCa---GC--------------ACGUGCGUCGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 10994 | 0.76 | 0.058613 |
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Target: 5'- gGCGCGCCUgggGGCGUCGUcGCcggaGCGGCAc -3' miRNA: 3'- -CGCGUGGG---CCGCAGCA-CGug--CGUCGU- -5' |
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| 411 | 3' | -60.8 | AC_000011.1 | + | 22368 | 1.1 | 0.000149 |
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Target: 5'- gGCGCACCCGGCGUCGUGCACGCAGCAg -3' miRNA: 3'- -CGCGUGGGCCGCAGCACGUGCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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