Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 416 | 3' | -58.1 | AC_000011.1 | + | 10897 | 0.7 | 0.246994 |
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Target: 5'- -uGUUGcGGugGagGGUGGUGGGGGCg -3' miRNA: 3'- acUAGCuCCugCagUCGCCGCUCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 26653 | 0.7 | 0.246994 |
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Target: 5'- gUGAUUGcGGGGCGU-GGCGGUGAGcGCu -3' miRNA: 3'- -ACUAGC-UCCUGCAgUCGCCGCUCcCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 10159 | 0.71 | 0.222407 |
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Target: 5'- cGGUaGAGcGGCcaucgcUCGGUGGCGGGGGCg -3' miRNA: 3'- aCUAgCUC-CUGc-----AGUCGCCGCUCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 17061 | 0.71 | 0.210912 |
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Target: 5'- cGGUgCGGcGACGgCGGCGGCGAGuGGUg -3' miRNA: 3'- aCUA-GCUcCUGCaGUCGCCGCUC-CCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 22555 | 0.72 | 0.205359 |
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Target: 5'- aUGAUCGAauGGAUGU-GGCGaGCGAgaacGGGCa -3' miRNA: 3'- -ACUAGCU--CCUGCAgUCGC-CGCU----CCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 25026 | 0.72 | 0.205359 |
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Target: 5'- --cUCGcGGAagGUCAGCGGCacgaaguccGAGGGCa -3' miRNA: 3'- acuAGCuCCUg-CAGUCGCCG---------CUCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 8460 | 0.73 | 0.174644 |
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Target: 5'- cGG-CGAGGACGcgCgccgGGCGGCaGGGGCg -3' miRNA: 3'- aCUaGCUCCUGCa-G----UCGCCGcUCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 84 | 0.79 | 0.06676 |
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Target: 5'- uUGGUCGAGGGaugagcgacCGUUAGgGGCG-GGGCg -3' miRNA: 3'- -ACUAGCUCCU---------GCAGUCgCCGCuCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 15749 | 0.79 | 0.061185 |
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Target: 5'- cGGUgGAgacGGGCG-CGGCGGCGGGGGUg -3' miRNA: 3'- aCUAgCU---CCUGCaGUCGCCGCUCCCG- -5' |
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| 416 | 3' | -58.1 | AC_000011.1 | + | 25060 | 1.1 | 0.000266 |
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Target: 5'- gUGAUCGAGGACGUCAGCGGCGAGGGCc -3' miRNA: 3'- -ACUAGCUCCUGCAGUCGCCGCUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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