Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4324 | 3' | -56.3 | NC_001720.1 | + | 12673 | 0.66 | 0.640877 |
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Target: 5'- cGuGAAagcCGGcUCGGACCCcuuaaagucuuccGGAguGAGCa -3' miRNA: 3'- uC-CUU---GCCaAGCCUGGG-------------CCUguCUCG- -5' |
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| 4324 | 3' | -56.3 | NC_001720.1 | + | 12389 | 0.66 | 0.598015 |
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Target: 5'- aAGGAcCGGUcgcgugCGGGCUggCGGcCGGGGCc -3' miRNA: 3'- -UCCUuGCCAa-----GCCUGG--GCCuGUCUCG- -5' |
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| 4324 | 3' | -56.3 | NC_001720.1 | + | 17279 | 0.67 | 0.565314 |
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Target: 5'- cGGucGCGGg--GGugCCGGACGaGGCg -3' miRNA: 3'- uCCu-UGCCaagCCugGGCCUGUcUCG- -5' |
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| 4324 | 3' | -56.3 | NC_001720.1 | + | 40187 | 0.67 | 0.533098 |
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Target: 5'- cGGAAUGGUUCccgccccaaaaaGGcaACCCGGugAGAc- -3' miRNA: 3'- uCCUUGCCAAG------------CC--UGGGCCugUCUcg -5' |
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| 4324 | 3' | -56.3 | NC_001720.1 | + | 18798 | 0.68 | 0.467853 |
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Target: 5'- cGGggUGGUUCccaacgucaaccugGGACCCGGccuCAGucaacuAGCa -3' miRNA: 3'- uCCuuGCCAAG--------------CCUGGGCCu--GUC------UCG- -5' |
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| 4324 | 3' | -56.3 | NC_001720.1 | + | 22755 | 0.69 | 0.460853 |
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Target: 5'- uGGAGCccaaGUUCGuGCCCGGA-GGGGCg -3' miRNA: 3'- uCCUUGc---CAAGCcUGGGCCUgUCUCG- -5' |
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| 4324 | 3' | -56.3 | NC_001720.1 | + | 24960 | 0.69 | 0.441163 |
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Target: 5'- gAGGAAgugcUGGccaucgUGGACCC-GACAGGGCg -3' miRNA: 3'- -UCCUU----GCCaa----GCCUGGGcCUGUCUCG- -5' |
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| 4324 | 3' | -56.3 | NC_001720.1 | + | 9958 | 0.69 | 0.431499 |
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Target: 5'- aGGGAGC-GUUCGGGguCUCGGGCuAGuGCa -3' miRNA: 3'- -UCCUUGcCAAGCCU--GGGCCUG-UCuCG- -5' |
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| 4324 | 3' | -56.3 | NC_001720.1 | + | 4259 | 1.11 | 0.000531 |
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Target: 5'- aAGGAACGGUUCGGACCCGGACAGAGCc -3' miRNA: 3'- -UCCUUGCCAAGCCUGGGCCUGUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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