miRNA display CGI


Results 21 - 26 of 26 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
4373 5' -50.7 NC_001720.1 + 12027 0.68 0.805759
Target:  5'- aGACCGUAAcgGGGGcGCGGGCagcggGAcagguaugcaccaccGGUCCg -3'
miRNA:   3'- aCUGGCGUU--UUUC-CGCCCG-----CU---------------UUAGG- -5'
4373 5' -50.7 NC_001720.1 + 33456 0.7 0.693451
Target:  5'- -cGCCGCGAAGAcaucgaagcGGCGGcGCGAcgaGGUCa -3'
miRNA:   3'- acUGGCGUUUUU---------CCGCC-CGCU---UUAGg -5'
4373 5' -50.7 NC_001720.1 + 22516 0.72 0.577354
Target:  5'- uUGACgauaGCu--GAGGCGGGCacgugcggaucgaGAAAUCCu -3'
miRNA:   3'- -ACUGg---CGuuuUUCCGCCCG-------------CUUUAGG- -5'
4373 5' -50.7 NC_001720.1 + 32086 0.76 0.360445
Target:  5'- gUGGCCGCGGAcauGGGcCGGGCGc-AUCUg -3'
miRNA:   3'- -ACUGGCGUUUu--UCC-GCCCGCuuUAGG- -5'
4373 5' -50.7 NC_001720.1 + 25793 0.77 0.317557
Target:  5'- -cGCCGCGGGugcGGCGGGCGGcucUCCg -3'
miRNA:   3'- acUGGCGUUUuu-CCGCCCGCUuu-AGG- -5'
4373 5' -50.7 NC_001720.1 + 19410 0.66 0.898257
Target:  5'- cGGCCGgGGccacGAGGGCGucccgcuCGAGAUCCu -3'
miRNA:   3'- aCUGGCgUU----UUUCCGCcc-----GCUUUAGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.