Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4373 | 5' | -50.7 | NC_001720.1 | + | 12027 | 0.68 | 0.805759 |
Target: 5'- aGACCGUAAcgGGGGcGCGGGCagcggGAcagguaugcaccaccGGUCCg -3' miRNA: 3'- aCUGGCGUU--UUUC-CGCCCG-----CU---------------UUAGG- -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 33456 | 0.7 | 0.693451 |
Target: 5'- -cGCCGCGAAGAcaucgaagcGGCGGcGCGAcgaGGUCa -3' miRNA: 3'- acUGGCGUUUUU---------CCGCC-CGCU---UUAGg -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 22516 | 0.72 | 0.577354 |
Target: 5'- uUGACgauaGCu--GAGGCGGGCacgugcggaucgaGAAAUCCu -3' miRNA: 3'- -ACUGg---CGuuuUUCCGCCCG-------------CUUUAGG- -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 32086 | 0.76 | 0.360445 |
Target: 5'- gUGGCCGCGGAcauGGGcCGGGCGc-AUCUg -3' miRNA: 3'- -ACUGGCGUUUu--UCC-GCCCGCuuUAGG- -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 25793 | 0.77 | 0.317557 |
Target: 5'- -cGCCGCGGGugcGGCGGGCGGcucUCCg -3' miRNA: 3'- acUGGCGUUUuu-CCGCCCGCUuu-AGG- -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 19410 | 0.66 | 0.898257 |
Target: 5'- cGGCCGgGGccacGAGGGCGucccgcuCGAGAUCCu -3' miRNA: 3'- aCUGGCgUU----UUUCCGCcc-----GCUUUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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