Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4373 | 5' | -50.7 | NC_001720.1 | + | 14356 | 0.67 | 0.845799 |
Target: 5'- gUGACUGUAAGAGGGgacugauaguucaGGGUGAcg-CCg -3' miRNA: 3'- -ACUGGCGUUUUUCCg------------CCCGCUuuaGG- -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 4944 | 0.66 | 0.875068 |
Target: 5'- uUGA-UGCu--GGGGUGGGCGcgGUCUa -3' miRNA: 3'- -ACUgGCGuuuUUCCGCCCGCuuUAGG- -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 11806 | 0.66 | 0.883082 |
Target: 5'- gUG-UCGCGcuuggGGGAGGCGGGUGGcagCCc -3' miRNA: 3'- -ACuGGCGU-----UUUUCCGCCCGCUuuaGG- -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 10865 | 0.66 | 0.890814 |
Target: 5'- cGACgCGCGAGAGucGCuGGUGAAcGUCCg -3' miRNA: 3'- aCUG-GCGUUUUUc-CGcCCGCUU-UAGG- -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 21642 | 0.66 | 0.890814 |
Target: 5'- --cCCGCAGGcGGcGuCGGGCGGAGcaggCCg -3' miRNA: 3'- acuGGCGUUUuUC-C-GCCCGCUUUa---GG- -5' |
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4373 | 5' | -50.7 | NC_001720.1 | + | 19410 | 0.66 | 0.898257 |
Target: 5'- cGGCCGgGGccacGAGGGCGucccgcuCGAGAUCCu -3' miRNA: 3'- aCUGGCgUU----UUUCCGCcc-----GCUUUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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