Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4373 | 5' | -50.7 | NC_001720.1 | + | 27554 | 0.73 | 0.500204 |
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Target: 5'- cGGCCGUugguuAGAGGGCGGaGCaGAAGaCCg -3' miRNA: 3'- aCUGGCGu----UUUUCCGCC-CG-CUUUaGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 19407 | 0.73 | 0.488304 |
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Target: 5'- cUGcCCGaCGGGcacGGGGCGGGCGAAgacaacgGUCCc -3' miRNA: 3'- -ACuGGC-GUUU---UUCCGCCCGCUU-------UAGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 14108 | 0.76 | 0.369493 |
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Target: 5'- gGAgCuCGGGGAGGCGGGCGcAAAUCUc -3' miRNA: 3'- aCUgGcGUUUUUCCGCCCGC-UUUAGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 32086 | 0.76 | 0.360445 |
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Target: 5'- gUGGCCGCGGAcauGGGcCGGGCGc-AUCUg -3' miRNA: 3'- -ACUGGCGUUUu--UCC-GCCCGCuuUAGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 25793 | 0.77 | 0.317557 |
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Target: 5'- -cGCCGCGGGugcGGCGGGCGGcucUCCg -3' miRNA: 3'- acUGGCGUUUuu-CCGCCCGCUuu-AGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 26692 | 1.13 | 0.001225 |
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Target: 5'- uUGACCGCAAAAAGGCGGGCGAAAUCCu -3' miRNA: 3'- -ACUGGCGUUUUUCCGCCCGCUUUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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