Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4373 | 5' | -50.7 | NC_001720.1 | + | 27554 | 0.73 | 0.500204 |
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Target: 5'- cGGCCGUugguuAGAGGGCGGaGCaGAAGaCCg -3' miRNA: 3'- aCUGGCGu----UUUUCCGCC-CG-CUUUaGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 32086 | 0.76 | 0.360445 |
|
Target: 5'- gUGGCCGCGGAcauGGGcCGGGCGc-AUCUg -3' miRNA: 3'- -ACUGGCGUUUu--UCC-GCCCGCuuUAGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 32207 | 0.67 | 0.840327 |
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Target: 5'- gUGGCuUGCAAGAAGGCGuccucauGCGAAcggCCu -3' miRNA: 3'- -ACUG-GCGUUUUUCCGCc------CGCUUua-GG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 32609 | 0.7 | 0.659094 |
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Target: 5'- aGACCGgGAAugGAGGgaaGGaacuGCGAGAUCCu -3' miRNA: 3'- aCUGGCgUUU--UUCCg--CC----CGCUUUAGG- -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 33456 | 0.7 | 0.693451 |
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Target: 5'- -cGCCGCGAAGAcaucgaagcGGCGGcGCGAcgaGGUCa -3' miRNA: 3'- acUGGCGUUUUU---------CCGCC-CGCU---UUAGg -5' |
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| 4373 | 5' | -50.7 | NC_001720.1 | + | 42740 | 0.67 | 0.840327 |
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Target: 5'- cGACCGUAAc-AGcCGGGCGGAAa-- -3' miRNA: 3'- aCUGGCGUUuuUCcGCCCGCUUUagg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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