Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5129 | 5' | -56.1 | NC_001798.1 | + | 5994 | 0.67 | 0.851616 |
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Target: 5'- -gGCUGCGCGGCggagaccgggACGGCaGCggCGg -3' miRNA: 3'- gaCGGCGCGCCGa---------UGCUGaUGaaGCa -5' |
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| 5129 | 5' | -56.1 | NC_001798.1 | + | 26499 | 0.67 | 0.843655 |
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Target: 5'- -gGCCGCGCGGggACGG-UGCUggccgCGg -3' miRNA: 3'- gaCGGCGCGCCgaUGCUgAUGAa----GCa -5' |
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| 5129 | 5' | -56.1 | NC_001798.1 | + | 116034 | 0.67 | 0.843655 |
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Target: 5'- -cGCgaUGCGCGGCguucgugaACGACUACUcgcuggucUCGUa -3' miRNA: 3'- gaCG--GCGCGCCGa-------UGCUGAUGA--------AGCA- -5' |
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| 5129 | 5' | -56.1 | NC_001798.1 | + | 112206 | 0.68 | 0.835502 |
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Target: 5'- -gGCCGCGCuGGCgga-GCUcGCUUCGUa -3' miRNA: 3'- gaCGGCGCG-CCGaugcUGA-UGAAGCA- -5' |
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| 5129 | 5' | -56.1 | NC_001798.1 | + | 98386 | 0.68 | 0.835502 |
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Target: 5'- -cGUCGagaGCGGCcccgACGGCUGCaUCGg -3' miRNA: 3'- gaCGGCg--CGCCGa---UGCUGAUGaAGCa -5' |
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| 5129 | 5' | -56.1 | NC_001798.1 | + | 134700 | 1.08 | 0.00348 |
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Target: 5'- gCUGCCGCGCGGCUACGACUACUUCGUg -3' miRNA: 3'- -GACGGCGCGCCGAUGCUGAUGAAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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