Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5155 | 5' | -56.3 | NC_001798.1 | + | 100844 | 0.66 | 0.895072 |
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Target: 5'- cGGGGCg----CUUGCCgUGGGGCUgCu -3' miRNA: 3'- cCCCUGacuaaGAGUGG-ACCCCGAgG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 104573 | 0.66 | 0.888445 |
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Target: 5'- cGGGuGCUGGUgUCUCggggaacgucGCCcGGGGCcgCCg -3' miRNA: 3'- -CCCcUGACUA-AGAG----------UGGaCCCCGa-GG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 67569 | 0.67 | 0.878796 |
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Target: 5'- gGGGGGCgaccucgggCUC-CC-GGGGUUCCu -3' miRNA: 3'- -CCCCUGacuaa----GAGuGGaCCCCGAGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 144585 | 0.67 | 0.867252 |
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Target: 5'- cGGGcGGCUGGg--UgGCgaGGGGCUUCg -3' miRNA: 3'- -CCC-CUGACUaagAgUGgaCCCCGAGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 102783 | 0.67 | 0.867252 |
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Target: 5'- gGGGGAUgug-UCauggCACCcGGGGCgCCg -3' miRNA: 3'- -CCCCUGacuaAGa---GUGGaCCCCGaGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 31062 | 0.67 | 0.859766 |
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Target: 5'- cGGGGGuCgGGcaCUaACCgGGGGCUCCc -3' miRNA: 3'- -CCCCU-GaCUaaGAgUGGaCCCCGAGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 118372 | 1.13 | 0.001524 |
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Target: 5'- gGGGGACUGAUUCUCACCUGGGGCUCCu -3' miRNA: 3'- -CCCCUGACUAAGAGUGGACCCCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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