Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5155 | 5' | -56.3 | NC_001798.1 | + | 100844 | 0.66 | 0.895072 |
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Target: 5'- cGGGGCg----CUUGCCgUGGGGCUgCu -3' miRNA: 3'- cCCCUGacuaaGAGUGG-ACCCCGAgG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 150597 | 0.66 | 0.900844 |
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Target: 5'- cGGGGgagagucGCUGAUgacUAUggGGGGCUCCu -3' miRNA: 3'- -CCCC-------UGACUAagaGUGgaCCCCGAGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 52091 | 0.66 | 0.901474 |
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Target: 5'- cGGGGcGCaUGAcguacaUCGCCaccGGGGCUCUg -3' miRNA: 3'- -CCCC-UG-ACUaag---AGUGGa--CCCCGAGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 15198 | 0.66 | 0.913591 |
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Target: 5'- gGGGGGCUGGcga--GCCgggGGGaGCgUCCg -3' miRNA: 3'- -CCCCUGACUaagagUGGa--CCC-CG-AGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 80673 | 0.66 | 0.913591 |
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Target: 5'- -uGGAcCUGGUUCUgGCCgcGGuGCUCCu -3' miRNA: 3'- ccCCU-GACUAAGAgUGGa-CCcCGAGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 151592 | 0.66 | 0.913591 |
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Target: 5'- uGGGGCUGcccuggcgCUCgGCCgGGGGCcgggCCg -3' miRNA: 3'- cCCCUGACuaa-----GAG-UGGaCCCCGa---GG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 112842 | 0.66 | 0.919302 |
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Target: 5'- uGGGugUGAaUUUCg---GGGGUUCCg -3' miRNA: 3'- cCCCugACUaAGAGuggaCCCCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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