Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5155 | 5' | -56.3 | NC_001798.1 | + | 141460 | 0.69 | 0.765664 |
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Target: 5'- cGGGGcugcgagcuGCUGAccggCUCGCCgcgcggGGGGCugaugUCCg -3' miRNA: 3'- -CCCC---------UGACUaa--GAGUGGa-----CCCCG-----AGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 56417 | 0.69 | 0.765664 |
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Target: 5'- cGGGGGCUGGUggCU-GCCgUGGuGCUCUa -3' miRNA: 3'- -CCCCUGACUAa-GAgUGG-ACCcCGAGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 36113 | 0.71 | 0.67675 |
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Target: 5'- gGGGGcccgGCUGcgUCUCGCCgcgaucccgccggUGGGGCg-- -3' miRNA: 3'- -CCCC----UGACuaAGAGUGG-------------ACCCCGagg -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 111841 | 0.72 | 0.590826 |
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Target: 5'- cGGGGcgggaaugccgucaaACaGGccgCUCACCaGGGGCUCCa -3' miRNA: 3'- -CCCC---------------UGaCUaa-GAGUGGaCCCCGAGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 73064 | 0.73 | 0.576759 |
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Target: 5'- uGGGGGcCUGGggggaCUacaGCCUGGGGCcCCu -3' miRNA: 3'- -CCCCU-GACUaa---GAg--UGGACCCCGaGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 150640 | 0.75 | 0.451763 |
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Target: 5'- cGGGGCUG--UCUCGCggGGGGCgUCCu -3' miRNA: 3'- cCCCUGACuaAGAGUGgaCCCCG-AGG- -5' |
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| 5155 | 5' | -56.3 | NC_001798.1 | + | 118372 | 1.13 | 0.001524 |
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Target: 5'- gGGGGACUGAUUCUCACCUGGGGCUCCu -3' miRNA: 3'- -CCCCUGACUAAGAGUGGACCCCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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