Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5505 | 5' | -56.3 | NC_001798.1 | + | 108053 | 0.67 | 0.834216 |
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Target: 5'- -cCGUGGGUacggacacccCCCCCCAucugcgGACUGGCAg- -3' miRNA: 3'- aaGCGUCUA----------GGGGGGU------UUGACCGUgu -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 111817 | 0.66 | 0.850663 |
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Target: 5'- gUCGCGGG-CgUCCagGGGCUGGCGCGg -3' miRNA: 3'- aAGCGUCUaGgGGGg-UUUGACCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 122390 | 0.71 | 0.608686 |
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Target: 5'- -cCGCGGcugUCCUCCGAGCUGGcCACGg -3' miRNA: 3'- aaGCGUCua-GGGGGGUUUGACC-GUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 129344 | 0.67 | 0.799113 |
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Target: 5'- -cCGCGGAgcgCCCgCCGGGcCUGGCGgGg -3' miRNA: 3'- aaGCGUCUa--GGGgGGUUU-GACCGUgU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 133585 | 0.67 | 0.834216 |
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Target: 5'- -aCGCAGAcgUUUCCCgGGGCggGGCGCGu -3' miRNA: 3'- aaGCGUCU--AGGGGGgUUUGa-CCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 136629 | 0.67 | 0.817011 |
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Target: 5'- -aCGCAGAgCUCUgCGGGgUGGCGCAa -3' miRNA: 3'- aaGCGUCUaGGGGgGUUUgACCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 151192 | 0.67 | 0.824843 |
|
Target: 5'- -gCGCGGAccgugugUCCCCCCAgcGACaggGaGCGCGg -3' miRNA: 3'- aaGCGUCU-------AGGGGGGU--UUGa--C-CGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 152624 | 0.72 | 0.516916 |
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Target: 5'- -aCGCAGAugggagUCCCCCCGGcgccccGCcGGCGCGg -3' miRNA: 3'- aaGCGUCU------AGGGGGGUU------UGaCCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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