Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5505 | 5' | -56.3 | NC_001798.1 | + | 36129 | 0.67 | 0.834216 |
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Target: 5'- cUCGCcgcGAUCCCgCCGGuggGGCGCGg -3' miRNA: 3'- aAGCGu--CUAGGGgGGUUugaCCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 133585 | 0.67 | 0.834216 |
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Target: 5'- -aCGCAGAcgUUUCCCgGGGCggGGCGCGu -3' miRNA: 3'- aaGCGUCU--AGGGGGgUUUGa-CCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 81738 | 0.66 | 0.839233 |
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Target: 5'- -gCGCAGGUCCCgCCGGACgcgugccaaaucGCGCGc -3' miRNA: 3'- aaGCGUCUAGGGgGGUUUGac----------CGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 20736 | 0.66 | 0.844997 |
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Target: 5'- -cCGaCAGAuccaaaaaauaccUCCCCCCGAGCUcccgcggggagcgacGGCGCc -3' miRNA: 3'- aaGC-GUCU-------------AGGGGGGUUUGA---------------CCGUGu -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 111817 | 0.66 | 0.850663 |
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Target: 5'- gUCGCGGG-CgUCCagGGGCUGGCGCGg -3' miRNA: 3'- aAGCGUCUaGgGGGg-UUUGACCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 73124 | 0.66 | 0.858584 |
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Target: 5'- -cCGUGGAccugUCUCCCCAGggGCUGGC-CGc -3' miRNA: 3'- aaGCGUCU----AGGGGGGUU--UGACCGuGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 23064 | 0.66 | 0.866294 |
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Target: 5'- gUUCGCAcGAUcccgucgccCCCCCCGGagcGCgacGGCGCGc -3' miRNA: 3'- -AAGCGU-CUA---------GGGGGGUU---UGa--CCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 1737 | 0.66 | 0.881055 |
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Target: 5'- -gCGCAGGUCCCgCgCCGccggccAGCgcacGGCGCAc -3' miRNA: 3'- aaGCGUCUAGGG-G-GGU------UUGa---CCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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