Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5505 | 5' | -56.3 | NC_001798.1 | + | 108053 | 0.67 | 0.834216 |
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Target: 5'- -cCGUGGGUacggacacccCCCCCCAucugcgGACUGGCAg- -3' miRNA: 3'- aaGCGUCUA----------GGGGGGU------UUGACCGUgu -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 70947 | 0.67 | 0.834216 |
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Target: 5'- -gCGguGGUCCCgCCGccgcuGCUGGCcCAg -3' miRNA: 3'- aaGCguCUAGGGgGGUu----UGACCGuGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 36129 | 0.67 | 0.834216 |
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Target: 5'- cUCGCcgcGAUCCCgCCGGuggGGCGCGg -3' miRNA: 3'- aAGCGu--CUAGGGgGGUUugaCCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 133585 | 0.67 | 0.834216 |
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Target: 5'- -aCGCAGAcgUUUCCCgGGGCggGGCGCGu -3' miRNA: 3'- aaGCGUCU--AGGGGGgUUUGa-CCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 75201 | 0.67 | 0.825704 |
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Target: 5'- -cCGCcccccGGAgCCCCCCGGcgccgacCUGGCGCAg -3' miRNA: 3'- aaGCG-----UCUaGGGGGGUUu------GACCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 76427 | 0.67 | 0.825704 |
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Target: 5'- -aCGCAag-CCCCUggaaGAGCUGGCGCGc -3' miRNA: 3'- aaGCGUcuaGGGGGg---UUUGACCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 151192 | 0.67 | 0.824843 |
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Target: 5'- -gCGCGGAccgugugUCCCCCCAgcGACaggGaGCGCGg -3' miRNA: 3'- aaGCGUCU-------AGGGGGGU--UUGa--C-CGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 28165 | 0.73 | 0.495261 |
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Target: 5'- -aCGCAGAgugggcgaacagCCCCCCGAGCgagGGCGgGg -3' miRNA: 3'- aaGCGUCUa-----------GGGGGGUUUGa--CCGUgU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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