Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5508 | 3' | -55.1 | NC_001798.1 | + | 57451 | 0.66 | 0.914989 |
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Target: 5'- cGGGGGCCCggucuugGGGGGCgGggCcgGUUUg -3' miRNA: 3'- aCCUCUGGGa------CCUCCGgUaaGa-CAAA- -5' |
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| 5508 | 3' | -55.1 | NC_001798.1 | + | 79228 | 0.66 | 0.914989 |
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Target: 5'- gGGAGaugaacuuugacGCCCUgGGGGGCCug-CUGg-- -3' miRNA: 3'- aCCUC------------UGGGA-CCUCCGGuaaGACaaa -5' |
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| 5508 | 3' | -55.1 | NC_001798.1 | + | 114494 | 0.66 | 0.908896 |
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Target: 5'- gGGAGGCCCUGGAcgggacGGgCAUUagcCUGc-- -3' miRNA: 3'- aCCUCUGGGACCU------CCgGUAA---GACaaa -5' |
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| 5508 | 3' | -55.1 | NC_001798.1 | + | 45004 | 0.66 | 0.908896 |
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Target: 5'- aGGAGGCCCUGGcgguGGUCAaugcgcUUUUGc-- -3' miRNA: 3'- aCCUCUGGGACCu---CCGGU------AAGACaaa -5' |
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| 5508 | 3' | -55.1 | NC_001798.1 | + | 78319 | 0.66 | 0.902556 |
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Target: 5'- cGGAauGACCC-GGAGGCCAccgcggagCUGg-- -3' miRNA: 3'- aCCU--CUGGGaCCUCCGGUaa------GACaaa -5' |
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| 5508 | 3' | -55.1 | NC_001798.1 | + | 90091 | 0.66 | 0.902556 |
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Target: 5'- cGGcAGGcCCCUGGAGGCgAgaucgCUGa-- -3' miRNA: 3'- aCC-UCU-GGGACCUCCGgUaa---GACaaa -5' |
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| 5508 | 3' | -55.1 | NC_001798.1 | + | 6219 | 0.66 | 0.902556 |
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Target: 5'- cGGuGACCCgGGGGGCCGg------- -3' miRNA: 3'- aCCuCUGGGaCCUCCGGUaagacaaa -5' |
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| 5508 | 3' | -55.1 | NC_001798.1 | + | 24623 | 0.66 | 0.895972 |
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Target: 5'- cGcGGGAUCCUGGAGGCg---CUGg-- -3' miRNA: 3'- aC-CUCUGGGACCUCCGguaaGACaaa -5' |
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| 5508 | 3' | -55.1 | NC_001798.1 | + | 14503 | 0.74 | 0.484738 |
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Target: 5'- cGGGGACCCgggugacuucgucUGGGGGCCuuucUCUGUg- -3' miRNA: 3'- aCCUCUGGG-------------ACCUCCGGua--AGACAaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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