Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5519 | 5' | -59.2 | NC_001798.1 | + | 33137 | 0.66 | 0.791631 |
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Target: 5'- -cCCCGCGCagGGGgGCcgggGAUGGGggGg -3' miRNA: 3'- gcGGGCGCGg-CCCgCGa---UUACUCuaC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 110526 | 0.66 | 0.791631 |
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Target: 5'- gCGUCCGCGCUGGGgGUcguuAUGAu--- -3' miRNA: 3'- -GCGGGCGCGGCCCgCGau--UACUcuac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 102638 | 0.66 | 0.791631 |
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Target: 5'- -uCCCGuCGCCGGGCgGCg---GAGGg- -3' miRNA: 3'- gcGGGC-GCGGCCCG-CGauuaCUCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 79444 | 0.66 | 0.791631 |
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Target: 5'- aCGCCCGCGCCcGGGCcuCUucuucccccGAGGg- -3' miRNA: 3'- -GCGGGCGCGG-CCCGc-GAuua------CUCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 40497 | 0.66 | 0.791631 |
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Target: 5'- uGCCUGUGCUGGGC-CggggGAGGg- -3' miRNA: 3'- gCGGGCGCGGCCCGcGauuaCUCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 25602 | 0.66 | 0.79252 |
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Target: 5'- gGgCCGCGCCGGGgGCgggccccccccgGAG-UGg -3' miRNA: 3'- gCgGGCGCGGCCCgCGauua--------CUCuAC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 71103 | 0.66 | 0.800461 |
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Target: 5'- gCGUgCGCGCCcccgGGGaCGCUGAUGGc--- -3' miRNA: 3'- -GCGgGCGCGG----CCC-GCGAUUACUcuac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 152268 | 0.66 | 0.800461 |
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Target: 5'- aGCUCGUGCCGGGCcgggGUUccAUGAGccGg -3' miRNA: 3'- gCGGGCGCGGCCCG----CGAu-UACUCuaC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 81257 | 0.66 | 0.800461 |
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Target: 5'- cCGCCCGCGacccgCGGGaccuuGCgggggGGGAUGg -3' miRNA: 3'- -GCGGGCGCg----GCCCg----CGauua-CUCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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