Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5519 | 5' | -59.2 | NC_001798.1 | + | 59700 | 0.67 | 0.716735 |
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Target: 5'- aCGCCCGUcuUCGGGCGCUu----GGUGg -3' miRNA: 3'- -GCGGGCGc-GGCCCGCGAuuacuCUAC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 31783 | 0.67 | 0.716735 |
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Target: 5'- aCGCgCGCgggucgggaggGCCGGGCGCggaggGAGGa- -3' miRNA: 3'- -GCGgGCG-----------CGGCCCGCGauua-CUCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 25730 | 0.67 | 0.72643 |
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Target: 5'- gCGCCCccgacgucucgGCGCUGGGCGCgca-GGGcGUGc -3' miRNA: 3'- -GCGGG-----------CGCGGCCCGCGauuaCUC-UAC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 66869 | 0.67 | 0.736047 |
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Target: 5'- uCGCCgggGCGCCGGG-GCU--UGGGAa- -3' miRNA: 3'- -GCGGg--CGCGGCCCgCGAuuACUCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 134995 | 0.67 | 0.736047 |
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Target: 5'- gGCCCGCGgUGGGCGCca--GGGu-- -3' miRNA: 3'- gCGGGCGCgGCCCGCGauuaCUCuac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 17121 | 0.67 | 0.736047 |
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Target: 5'- cCGCCCGCGagCGGuaguGCGC-GGUGAGGc- -3' miRNA: 3'- -GCGGGCGCg-GCC----CGCGaUUACUCUac -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 148016 | 0.67 | 0.742729 |
|
Target: 5'- gCGCCCGUccccuuccucuaccGCgUGGGCGCgggcgggGGGGUGg -3' miRNA: 3'- -GCGGGCG--------------CG-GCCCGCGauua---CUCUAC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 91786 | 0.67 | 0.745578 |
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Target: 5'- aCGCCCccugccgaugacGCGCgGGGCGUggggaccGUGGGcgGg -3' miRNA: 3'- -GCGGG------------CGCGgCCCGCGau-----UACUCuaC- -5' |
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| 5519 | 5' | -59.2 | NC_001798.1 | + | 81257 | 0.66 | 0.800461 |
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Target: 5'- cCGCCCGCGacccgCGGGaccuuGCgggggGGGAUGg -3' miRNA: 3'- -GCGGGCGCg----GCCCg----CGauua-CUCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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