Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5521 | 5' | -59.8 | NC_001798.1 | + | 135773 | 0.66 | 0.748234 |
Target: 5'- cGGCCggcCGccGGcccgccGGAgGAGGCCGUg -3' miRNA: 3'- aCCGGa--GCuaCCa-----CCUgCUCCGGCAa -5' |
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5521 | 5' | -59.8 | NC_001798.1 | + | 139168 | 0.67 | 0.689876 |
Target: 5'- cGGCCUCGcUGGaccGGACGAcGuuGUa -3' miRNA: 3'- aCCGGAGCuACCa--CCUGCUcCggCAa -5' |
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5521 | 5' | -59.8 | NC_001798.1 | + | 140508 | 0.68 | 0.609761 |
Target: 5'- cGGCCaaccuucCGG-GGUGGACGcGGCUGUUc -3' miRNA: 3'- aCCGGa------GCUaCCACCUGCuCCGGCAA- -5' |
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5521 | 5' | -59.8 | NC_001798.1 | + | 144075 | 0.66 | 0.709605 |
Target: 5'- cUGGCCggGAUGGUGGuACGGGuCCu-- -3' miRNA: 3'- -ACCGGagCUACCACC-UGCUCcGGcaa -5' |
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5521 | 5' | -59.8 | NC_001798.1 | + | 148840 | 0.71 | 0.428819 |
Target: 5'- aGGCCUCGggGGUGGGgGcGGCUu-- -3' miRNA: 3'- aCCGGAGCuaCCACCUgCuCCGGcaa -5' |
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5521 | 5' | -59.8 | NC_001798.1 | + | 151167 | 0.67 | 0.64993 |
Target: 5'- gGGCCaCGGgggGGUGGGCGAcagGGCgCGg- -3' miRNA: 3'- aCCGGaGCUa--CCACCUGCU---CCG-GCaa -5' |
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5521 | 5' | -59.8 | NC_001798.1 | + | 151602 | 0.66 | 0.748234 |
Target: 5'- cUGGCgCUCGGccGG-GGGCcGGGCCGg- -3' miRNA: 3'- -ACCG-GAGCUa-CCaCCUGcUCCGGCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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