Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5521 | 5' | -59.8 | NC_001798.1 | + | 151167 | 0.67 | 0.64993 |
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Target: 5'- gGGCCaCGGgggGGUGGGCGAcagGGCgCGg- -3' miRNA: 3'- aCCGGaGCUa--CCACCUGCU---CCG-GCaa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 90164 | 0.67 | 0.679938 |
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Target: 5'- aUGuGCUcgagggCGGUGGUGGAC-AGGUCGUa -3' miRNA: 3'- -AC-CGGa-----GCUACCACCUGcUCCGGCAa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 121788 | 0.67 | 0.679938 |
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Target: 5'- cGGUCUCGGgcgGGgccGGGCc-GGCCGUUg -3' miRNA: 3'- aCCGGAGCUa--CCa--CCUGcuCCGGCAA- -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 134989 | 0.67 | 0.689876 |
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Target: 5'- gGGCCUgGcccgcGGUGGGCGccaGGGUCGUc -3' miRNA: 3'- aCCGGAgCua---CCACCUGC---UCCGGCAa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 139168 | 0.67 | 0.689876 |
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Target: 5'- cGGCCUCGcUGGaccGGACGAcGuuGUa -3' miRNA: 3'- aCCGGAGCuACCa--CCUGCUcCggCAa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 144075 | 0.66 | 0.709605 |
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Target: 5'- cUGGCCggGAUGGUGGuACGGGuCCu-- -3' miRNA: 3'- -ACCGGagCUACCACC-UGCUCcGGcaa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 95563 | 0.66 | 0.757667 |
|
Target: 5'- cGGCCcUGGaGGUGGcccACGAGGCgGa- -3' miRNA: 3'- aCCGGaGCUaCCACC---UGCUCCGgCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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