Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5521 | 5' | -59.8 | NC_001798.1 | + | 135773 | 0.66 | 0.748234 |
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Target: 5'- cGGCCggcCGccGGcccgccGGAgGAGGCCGUg -3' miRNA: 3'- aCCGGa--GCuaCCa-----CCUgCUCCGGCAa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 33274 | 0.66 | 0.748234 |
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Target: 5'- -uGUCUUGGUgGGUGGGCG-GGCUGg- -3' miRNA: 3'- acCGGAGCUA-CCACCUGCuCCGGCaa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 151602 | 0.66 | 0.748234 |
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Target: 5'- cUGGCgCUCGGccGG-GGGCcGGGCCGg- -3' miRNA: 3'- -ACCG-GAGCUa-CCaCCUGcUCCGGCaa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 79862 | 0.66 | 0.748234 |
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Target: 5'- gGcGCCUCGAUGGac-GCGGcGGCCGa- -3' miRNA: 3'- aC-CGGAGCUACCaccUGCU-CCGGCaa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 130531 | 0.66 | 0.757667 |
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Target: 5'- gUGGCggCGAUGGccccgaGGugGGGGCCc-- -3' miRNA: 3'- -ACCGgaGCUACCa-----CCugCUCCGGcaa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 26534 | 0.66 | 0.757667 |
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Target: 5'- cGGCgUggaGGUGGUGGggaccgccGCGGGGCUGg- -3' miRNA: 3'- aCCGgAg--CUACCACC--------UGCUCCGGCaa -5' |
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| 5521 | 5' | -59.8 | NC_001798.1 | + | 95563 | 0.66 | 0.757667 |
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Target: 5'- cGGCCcUGGaGGUGGcccACGAGGCgGa- -3' miRNA: 3'- aCCGGaGCUaCCACC---UGCUCCGgCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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