Results 1 - 20 of 69 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5542 | 5' | -67.5 | NC_001806.1 | + | 3344 | 0.66 | 0.41197 |
Target: 5'- -gGCCGCGGggggcuggCgggCCGGGCCCCggCCa- -3' miRNA: 3'- caCGGCGCCga------Ga--GGCCCGGGG--GGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 78280 | 0.66 | 0.41197 |
Target: 5'- -cGCCGCGGCaaguUgUCCGaaaccgaCCCCCUGg -3' miRNA: 3'- caCGGCGCCG----AgAGGCccg----GGGGGAC- -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 49645 | 0.66 | 0.403913 |
Target: 5'- cGUGCCG-GGCccggagCUCCGGGCggcauuCCCgUUGg -3' miRNA: 3'- -CACGGCgCCGa-----GAGGCCCG------GGGgGAC- -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 72408 | 0.66 | 0.402313 |
Target: 5'- -gGCCGCGGCUCUgcaucuuuaCgGGGCCagcgagacuuaCCUGc -3' miRNA: 3'- caCGGCGCCGAGA---------GgCCCGGgg---------GGAC- -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 84881 | 0.66 | 0.393588 |
Target: 5'- -gGCCgGCGGgaCUCugggcguggugcguCGGGCCUCCCg- -3' miRNA: 3'- caCGG-CGCCgaGAG--------------GCCCGGGGGGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 23372 | 0.66 | 0.3881 |
Target: 5'- -cGCCccCGGCUCggacgcCCGcGCCCCCCUc -3' miRNA: 3'- caCGGc-GCCGAGa-----GGCcCGGGGGGAc -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 146839 | 0.66 | 0.3881 |
Target: 5'- gGUGCgGCGGCUCcagCCGGGgaCCgcggCCCg- -3' miRNA: 3'- -CACGgCGCCGAGa--GGCCCg-GG----GGGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 146198 | 0.66 | 0.380348 |
Target: 5'- -gGCCGCGGCUCUgUGGuuaacaccagaGCCUgCCCa- -3' miRNA: 3'- caCGGCGCCGAGAgGCC-----------CGGG-GGGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 16499 | 0.66 | 0.380348 |
Target: 5'- -cGCCGCGGCUga-UGGcucgagccGCCUCCCUGu -3' miRNA: 3'- caCGGCGCCGAgagGCC--------CGGGGGGAC- -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 38662 | 0.66 | 0.371941 |
Target: 5'- gGUGCCGgggcCGGCUCUCCcaaaccccuccucGuuccGCCCCCCg- -3' miRNA: 3'- -CACGGC----GCCGAGAGG-------------Cc---CGGGGGGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 149656 | 0.66 | 0.365157 |
Target: 5'- -cGCC-CGGCUC-CCguguaugaguggGGGUCCCCCa- -3' miRNA: 3'- caCGGcGCCGAGaGG------------CCCGGGGGGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 29961 | 0.67 | 0.35772 |
Target: 5'- -aGCCGCGGCg--CCGcggggggcgucuGGCCCCuCCg- -3' miRNA: 3'- caCGGCGCCGagaGGC------------CCGGGG-GGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 39190 | 0.67 | 0.356982 |
Target: 5'- -gGCgGCGGCcuugUCUgcguucuugggggCCGGGCCCCgCCg- -3' miRNA: 3'- caCGgCGCCG----AGA-------------GGCCCGGGG-GGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 94175 | 0.67 | 0.35039 |
Target: 5'- -cGCUGCGGCgUCgucugaggCCGGGCCUggaggCCCa- -3' miRNA: 3'- caCGGCGCCG-AGa-------GGCCCGGG-----GGGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 73945 | 0.67 | 0.35039 |
Target: 5'- -cGaCGCGGuCUCacagcacgCCGGGCCCCUCg- -3' miRNA: 3'- caCgGCGCC-GAGa-------GGCCCGGGGGGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 33135 | 0.67 | 0.35039 |
Target: 5'- -cGCCGCGG-UCgg-GGGCCCCUCg- -3' miRNA: 3'- caCGGCGCCgAGaggCCCGGGGGGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 31769 | 0.67 | 0.343168 |
Target: 5'- -aGCCGCGGC---CCGGGCUgCCUg- -3' miRNA: 3'- caCGGCGCCGagaGGCCCGGgGGGac -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 32680 | 0.67 | 0.336053 |
Target: 5'- aGUGgCGUGGCcgaccuuagcCUCUgGGGCgCCCCCUGu -3' miRNA: 3'- -CACgGCGCCGa---------GAGG-CCCG-GGGGGAC- -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 135780 | 0.67 | 0.336053 |
Target: 5'- -aGCUGCGGCgC-CCGc-CCCCCCUGg -3' miRNA: 3'- caCGGCGCCGaGaGGCccGGGGGGAC- -5' |
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5542 | 5' | -67.5 | NC_001806.1 | + | 6829 | 0.67 | 0.331837 |
Target: 5'- cUGCUGC-GCUCUcCCGGGCugcugcccgaaaucgCCCCCa- -3' miRNA: 3'- cACGGCGcCGAGA-GGCCCG---------------GGGGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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