Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5546 | 5' | -60 | NC_001806.1 | + | 151063 | 0.7 | 0.512011 |
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Target: 5'- cGCaCCCGCGC--GGgGGUCgcgGGGGUCGc -3' miRNA: 3'- -CG-GGGCGCGaaCUgCCAG---UCCCAGCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 4970 | 0.7 | 0.51957 |
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Target: 5'- cGUCCCGCcggGCgucgucgagucgUGGgggUGGUCGGGGUCGUg -3' miRNA: 3'- -CGGGGCG---CGa-----------ACU---GCCAGUCCCAGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 20979 | 0.69 | 0.559916 |
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Target: 5'- aGUUCCGCGUc-GACGGcgGGGGUCGUc -3' miRNA: 3'- -CGGGGCGCGaaCUGCCagUCCCAGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 5060 | 0.69 | 0.589261 |
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Target: 5'- gGCCCCcCGCUcGucGCGGUCuGGGcUCGg -3' miRNA: 3'- -CGGGGcGCGAaC--UGCCAGuCCC-AGCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 3485 | 0.69 | 0.606017 |
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Target: 5'- cGCCCgGCGUcgUcgucggcgucggcgUGGCGGgcggcgUCGGGGUCGUc -3' miRNA: 3'- -CGGGgCGCG--A--------------ACUGCC------AGUCCCAGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 20661 | 0.69 | 0.60898 |
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Target: 5'- gGCCCCcgGCGUgccgGcguCGGggCGGGGUCGUg -3' miRNA: 3'- -CGGGG--CGCGaa--Cu--GCCa-GUCCCAGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 41215 | 0.68 | 0.618867 |
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Target: 5'- aGCCCCGCGCcuaaaGugGccCAGGGccUCGUg -3' miRNA: 3'- -CGGGGCGCGaa---CugCcaGUCCC--AGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 5442 | 0.66 | 0.782189 |
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Target: 5'- cGCUCCGUGUg-GACGaUCGGGG-CGUc -3' miRNA: 3'- -CGGGGCGCGaaCUGCcAGUCCCaGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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