Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5546 | 5' | -60 | NC_001806.1 | + | 84233 | 0.67 | 0.707396 |
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Target: 5'- gGCCaCCGUGCUgaaacACGGcCGGGGgcgCGg -3' miRNA: 3'- -CGG-GGCGCGAac---UGCCaGUCCCa--GCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 144348 | 0.67 | 0.717041 |
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Target: 5'- cGCCCCGCGC----CGGUCcauuaAGGGcgcgCGUg -3' miRNA: 3'- -CGGGGCGCGaacuGCCAG-----UCCCa---GCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 41815 | 0.67 | 0.726618 |
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Target: 5'- gGCCCCGCaauacGCgggGGCGGgcgUCAGGGcccCGg -3' miRNA: 3'- -CGGGGCG-----CGaa-CUGCC---AGUCCCa--GCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 24067 | 0.66 | 0.745536 |
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Target: 5'- cGCCCCcgacgugucgGCGCUgGGCGcaCAGGG-CGUg -3' miRNA: 3'- -CGGGG----------CGCGAaCUGCcaGUCCCaGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 88809 | 0.66 | 0.754859 |
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Target: 5'- gGCCCaggaGCGUgUGACGGUCcccGUCGg -3' miRNA: 3'- -CGGGg---CGCGaACUGCCAGuccCAGCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 740 | 0.66 | 0.754859 |
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Target: 5'- cGUCCCGCGCUcccuCGGg-GGGGuUCGg -3' miRNA: 3'- -CGGGGCGCGAacu-GCCagUCCC-AGCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 149793 | 0.66 | 0.764081 |
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Target: 5'- aCCUCGCGCUgggGGCGGcccUCAGGccggCGg -3' miRNA: 3'- cGGGGCGCGAa--CUGCC---AGUCCca--GCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 5442 | 0.66 | 0.782189 |
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Target: 5'- cGCUCCGUGUg-GACGaUCGGGG-CGUc -3' miRNA: 3'- -CGGGGCGCGaaCUGCcAGUCCCaGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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