Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5550 | 5' | -54.5 | NC_001806.1 | + | 54774 | 0.66 | 0.935615 |
Target: 5'- aUGGUU--GGCCGCGGcaGCACCAg--- -3' miRNA: 3'- cACCAGacCUGGCGCC--UGUGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 36246 | 0.66 | 0.925099 |
Target: 5'- -aGGUgCUguaGGGCCGCGGAgGCCc---- -3' miRNA: 3'- caCCA-GA---CCUGGCGCCUgUGGuaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 119721 | 0.66 | 0.925099 |
Target: 5'- aGUGG-UUGGAUCGCGGAUgggaagcccugGCCGg--- -3' miRNA: 3'- -CACCaGACCUGGCGCCUG-----------UGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 37527 | 0.66 | 0.925099 |
Target: 5'- -aGG-CUcGGGCCGUGGGCgACCAa--- -3' miRNA: 3'- caCCaGA-CCUGGCGCCUG-UGGUaaau -5' |
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5550 | 5' | -54.5 | NC_001806.1 | + | 132650 | 0.66 | 0.945141 |
Target: 5'- cUGGUgCgcgGGGCCGCGGccgcuuACGCCGc--- -3' miRNA: 3'- cACCA-Ga--CCUGGCGCC------UGUGGUaaau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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