Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5555 | 5' | -56.8 | NC_001806.1 | + | 107265 | 0.75 | 0.380826 |
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Target: 5'- ---aCGCCCUguaUGCCGAGuuUCUAGGGGGc -3' miRNA: 3'- aaagGCGGGA---ACGGCUU--AGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 132524 | 0.67 | 0.806107 |
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Target: 5'- --cCCGUCUUUGCCuuucuggggcccGAguuugagGUCCGGGGGGg -3' miRNA: 3'- aaaGGCGGGAACGG------------CU-------UAGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 132907 | 0.69 | 0.711484 |
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Target: 5'- --gCgGCCCUgGCCGAccUCGGGGGGa -3' miRNA: 3'- aaaGgCGGGAaCGGCUuaGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 135827 | 1.07 | 0.002837 |
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Target: 5'- gUUUCCGCCCUUGCCGAAUCCAGGGAGg -3' miRNA: 3'- -AAAGGCGGGAACGGCUUAGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 137067 | 0.67 | 0.798021 |
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Target: 5'- --aCCGaCCa--GCCGu-UCCAGGGAGg -3' miRNA: 3'- aaaGGC-GGgaaCGGCuuAGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 137577 | 0.7 | 0.639979 |
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Target: 5'- ---gCGCCCUgGCCGccUCCGGGGGc -3' miRNA: 3'- aaagGCGGGAaCGGCuuAGGUCCCUc -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 147362 | 0.74 | 0.397703 |
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Target: 5'- --gCCGCCCUcuuggccccUGCCGGcgCgAGGGGGg -3' miRNA: 3'- aaaGGCGGGA---------ACGGCUuaGgUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 147676 | 0.69 | 0.701401 |
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Target: 5'- -cUCCGCCCcggggGCCGGggCgCGGGGGc -3' miRNA: 3'- aaAGGCGGGaa---CGGCUuaG-GUCCCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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