Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5555 | 5' | -56.8 | NC_001806.1 | + | 87879 | 0.67 | 0.82446 |
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Target: 5'- --cCCGgCCguucaugcgGCCGuacccGUCCAGGGGGa -3' miRNA: 3'- aaaGGCgGGaa-------CGGCu----UAGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 103197 | 0.66 | 0.841212 |
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Target: 5'- -cUCCGCC---GCCGucUCCcgGGGGAGg -3' miRNA: 3'- aaAGGCGGgaaCGGCuuAGG--UCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 22115 | 0.66 | 0.841212 |
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Target: 5'- --gCCGCCCggGCC---UCUGGGGGGc -3' miRNA: 3'- aaaGGCGGGaaCGGcuuAGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 23262 | 0.66 | 0.841212 |
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Target: 5'- --gCCGCCUccGCCGcg-CCGcGGGAGg -3' miRNA: 3'- aaaGGCGGGaaCGGCuuaGGU-CCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 2710 | 0.66 | 0.864879 |
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Target: 5'- --gCCGCCa--GCCGc--CCAGGGGGu -3' miRNA: 3'- aaaGGCGGgaaCGGCuuaGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 19344 | 0.66 | 0.872351 |
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Target: 5'- --aCCGCCCcccaaGCC---UCCGGGGGGc -3' miRNA: 3'- aaaGGCGGGaa---CGGcuuAGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 51111 | 0.66 | 0.872351 |
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Target: 5'- --gCCGCCCUgcgggcGCaCGug-UCGGGGAGg -3' miRNA: 3'- aaaGGCGGGAa-----CG-GCuuaGGUCCCUC- -5' |
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| 5555 | 5' | -56.8 | NC_001806.1 | + | 64963 | 0.66 | 0.872351 |
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Target: 5'- ---gCGCCCggGCCGGggGUCCcGGGGc -3' miRNA: 3'- aaagGCGGGaaCGGCU--UAGGuCCCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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