Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5555 | 5' | -56.8 | NC_001806.1 | + | 83310 | 0.7 | 0.629671 |
Target: 5'- --cCCGCCC---CCGGAUCCcuguGGGGAGg -3' miRNA: 3'- aaaGGCGGGaacGGCUUAGG----UCCCUC- -5' |
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5555 | 5' | -56.8 | NC_001806.1 | + | 57352 | 0.7 | 0.629671 |
Target: 5'- --cCCGuCCCUgGCCGuccUCCGGGGAc -3' miRNA: 3'- aaaGGC-GGGAaCGGCuu-AGGUCCCUc -5' |
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5555 | 5' | -56.8 | NC_001806.1 | + | 137577 | 0.7 | 0.639979 |
Target: 5'- ---gCGCCCUgGCCGccUCCGGGGGc -3' miRNA: 3'- aaagGCGGGAaCGGCuuAGGUCCCUc -5' |
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5555 | 5' | -56.8 | NC_001806.1 | + | 26635 | 0.69 | 0.681067 |
Target: 5'- --cCCGCCCgaGCCGGcgCCAGaGGc- -3' miRNA: 3'- aaaGGCGGGaaCGGCUuaGGUC-CCuc -5' |
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5555 | 5' | -56.8 | NC_001806.1 | + | 70329 | 0.69 | 0.681067 |
Target: 5'- ---aCGCCCUgGCCGc--CCGGGGGGu -3' miRNA: 3'- aaagGCGGGAaCGGCuuaGGUCCCUC- -5' |
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5555 | 5' | -56.8 | NC_001806.1 | + | 147676 | 0.69 | 0.701401 |
Target: 5'- -cUCCGCCCcggggGCCGGggCgCGGGGGc -3' miRNA: 3'- aaAGGCGGGaa---CGGCUuaG-GUCCCUc -5' |
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5555 | 5' | -56.8 | NC_001806.1 | + | 132907 | 0.69 | 0.711484 |
Target: 5'- --gCgGCCCUgGCCGAccUCGGGGGGa -3' miRNA: 3'- aaaGgCGGGAaCGGCUuaGGUCCCUC- -5' |
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5555 | 5' | -56.8 | NC_001806.1 | + | 64963 | 0.66 | 0.872351 |
Target: 5'- ---gCGCCCggGCCGGggGUCCcGGGGc -3' miRNA: 3'- aaagGCGGGaaCGGCU--UAGGuCCCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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