Results 1 - 20 of 92 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5559 | 3' | -62.5 | NC_001806.1 | + | 30835 | 0.66 | 0.643519 |
Target: 5'- aCCUCCCUcggcCCCCGCGCuGcuucUGGGccGCGg -3' miRNA: 3'- cGGGGGGA----GGGGCGUGuCu---ACCC--UGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 79923 | 0.66 | 0.662962 |
Target: 5'- cGCCCCCggucgcaaCCgGCACGGuuuugcccgGGGGCGa -3' miRNA: 3'- -CGGGGGgag-----GGgCGUGUCua-------CCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 20302 | 0.66 | 0.661992 |
Target: 5'- cGCCCCCUUggggcggUCCCGCccgccggccaAUGGG-GGGGCGg -3' miRNA: 3'- -CGGGGGGA-------GGGGCG----------UGUCUaCCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 60979 | 0.66 | 0.666842 |
Target: 5'- gGCCCCCC-CaCCCGCGuucuacaaggacgucCuGGccaaauucUGGGACGa -3' miRNA: 3'- -CGGGGGGaG-GGGCGU---------------GuCU--------ACCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 59002 | 0.66 | 0.643519 |
Target: 5'- gGCgCCCCCUCCuCCG-GCugcccGA-GGGACGc -3' miRNA: 3'- -CG-GGGGGAGG-GGCgUGu----CUaCCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 100378 | 0.66 | 0.643519 |
Target: 5'- aGCUCCCgUCgCCGgGCGGcgcgGGGGCc -3' miRNA: 3'- -CGGGGGgAGgGGCgUGUCua--CCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 21429 | 0.66 | 0.621123 |
Target: 5'- cGCCgCCCCggacCCCCGCcuuucgagccgacgACGauGAcgGGGACGa -3' miRNA: 3'- -CGG-GGGGa---GGGGCG--------------UGU--CUa-CCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 55270 | 0.66 | 0.652276 |
Target: 5'- gGCCCCCUccgCCCagaGCACguggggguccguuAGGUugGGGGCGc -3' miRNA: 3'- -CGGGGGGa--GGGg--CGUG-------------UCUA--CCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 46364 | 0.66 | 0.662962 |
Target: 5'- cGCCCCCCcaaaCCCCGaCGCgccauGGAccccccGGGugGc -3' miRNA: 3'- -CGGGGGGa---GGGGC-GUG-----UCUa-----CCCugC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 30770 | 0.66 | 0.662962 |
Target: 5'- cGUCCUCCUCCgcuuCCGCcuccuCGGgcGGGGCc -3' miRNA: 3'- -CGGGGGGAGG----GGCGu----GUCuaCCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 720 | 0.66 | 0.647412 |
Target: 5'- gGCCCcagCCCUCCCCGgCGCgucccgcgcucccucGGggGGGuuCGg -3' miRNA: 3'- -CGGG---GGGAGGGGC-GUG---------------UCuaCCCu-GC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 140338 | 0.66 | 0.633782 |
Target: 5'- uGCCCCCCcCCCCuuGCGGgcGGucCa -3' miRNA: 3'- -CGGGGGGaGGGGcgUGUCuaCCcuGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 47234 | 0.66 | 0.633782 |
Target: 5'- gGCCCCCC-CgCUGUACGcaacGGGGCGc -3' miRNA: 3'- -CGGGGGGaGgGGCGUGUcua-CCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 73963 | 0.66 | 0.653248 |
Target: 5'- cGCCgggCCCCUCggcguaaugCCCGCccCGGccGGGGCGg -3' miRNA: 3'- -CGG---GGGGAG---------GGGCGu-GUCuaCCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 5060 | 0.66 | 0.633782 |
Target: 5'- gGCCCCCCg-CUCGuCGCGGucugggcucgGGGugGg -3' miRNA: 3'- -CGGGGGGagGGGC-GUGUCua--------CCCugC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 41354 | 0.66 | 0.653248 |
Target: 5'- uGCCCCCCggcaUCCaggCCGC-CAG--GGGGCa -3' miRNA: 3'- -CGGGGGG----AGG---GGCGuGUCuaCCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 23782 | 0.66 | 0.624044 |
Target: 5'- -aCCCCCaCCCC-CACGGGgccgccgGGGGCc -3' miRNA: 3'- cgGGGGGaGGGGcGUGUCUa------CCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 115820 | 0.66 | 0.662962 |
Target: 5'- cGCCCCCUuugUCCCCGCcgACAagccgcgauuccGA-GGGcCGg -3' miRNA: 3'- -CGGGGGG---AGGGGCG--UGU------------CUaCCCuGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 22070 | 0.66 | 0.63086 |
Target: 5'- gGCCCCCgC-CCCCgGgGCGGGUGcuguacggcggccuGGGCGa -3' miRNA: 3'- -CGGGGG-GaGGGG-CgUGUCUAC--------------CCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 26888 | 0.66 | 0.624044 |
Target: 5'- -aCCCCCgcgacCCCCGCGCGGGUGcG-Cu -3' miRNA: 3'- cgGGGGGa----GGGGCGUGUCUACcCuGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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