Results 1 - 20 of 92 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5559 | 3' | -62.5 | NC_001806.1 | + | 720 | 0.66 | 0.647412 |
Target: 5'- gGCCCcagCCCUCCCCGgCGCgucccgcgcucccucGGggGGGuuCGg -3' miRNA: 3'- -CGGG---GGGAGGGGC-GUG---------------UCuaCCCu-GC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 2455 | 0.73 | 0.287726 |
Target: 5'- gGCCCCCggcggCCCCGUGgGGGUGGGGg- -3' miRNA: 3'- -CGGGGGga---GGGGCGUgUCUACCCUgc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 2974 | 0.7 | 0.388301 |
Target: 5'- uGCgCCCCUCCCgCgGCGCGGGgaGGcGGCGg -3' miRNA: 3'- -CGgGGGGAGGG-G-CGUGUCUa-CC-CUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 5060 | 0.66 | 0.633782 |
Target: 5'- gGCCCCCCg-CUCGuCGCGGucugggcucgGGGugGg -3' miRNA: 3'- -CGGGGGGagGGGC-GUGUCua--------CCCugC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 5850 | 0.71 | 0.357332 |
Target: 5'- gGCCCCCCggUCCCGCccgcCGGAcgccGGGACc -3' miRNA: 3'- -CGGGGGGa-GGGGCGu---GUCUa---CCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 5910 | 0.67 | 0.563079 |
Target: 5'- cGCCCgCCUUgCCGCccccccauuggccgGCGGgcGGGACc -3' miRNA: 3'- -CGGGgGGAGgGGCG--------------UGUCuaCCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 14113 | 0.7 | 0.404432 |
Target: 5'- uGCCCCCCcauccCCCUGC-CGGAcaugguuuggGGGGCGc -3' miRNA: 3'- -CGGGGGGa----GGGGCGuGUCUa---------CCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 17665 | 0.72 | 0.335268 |
Target: 5'- aGCCCCCCUCgCCUcuacCACuuGUGGGugGu -3' miRNA: 3'- -CGGGGGGAG-GGGc---GUGucUACCCugC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 18247 | 0.68 | 0.500139 |
Target: 5'- uCCgCCCUUCCCGCcauuaAGAcgcUGGGACa -3' miRNA: 3'- cGGgGGGAGGGGCGug---UCU---ACCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 20160 | 0.7 | 0.407709 |
Target: 5'- cGCCCCCCgccgcccgggcccgCCCCcggggccgGCGCGGAgucGGGcACGg -3' miRNA: 3'- -CGGGGGGa-------------GGGG--------CGUGUCUa--CCC-UGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 20302 | 0.66 | 0.661992 |
Target: 5'- cGCCCCCUUggggcggUCCCGCccgccggccaAUGGG-GGGGCGg -3' miRNA: 3'- -CGGGGGGA-------GGGGCG----------UGUCUaCCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 20586 | 0.72 | 0.307425 |
Target: 5'- cGCCCUCgCgCCCCGCccaugGCAGAUGGcGCGg -3' miRNA: 3'- -CGGGGG-GaGGGGCG-----UGUCUACCcUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 20756 | 0.71 | 0.349866 |
Target: 5'- uGCuCCUCCUUCCCGC-CGGccccUGGGACu -3' miRNA: 3'- -CG-GGGGGAGGGGCGuGUCu---ACCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 21429 | 0.66 | 0.621123 |
Target: 5'- cGCCgCCCCggacCCCCGCcuuucgagccgacgACGauGAcgGGGACGa -3' miRNA: 3'- -CGG-GGGGa---GGGGCG--------------UGU--CUa-CCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 22070 | 0.66 | 0.63086 |
Target: 5'- gGCCCCCgC-CCCCgGgGCGGGUGcuguacggcggccuGGGCGa -3' miRNA: 3'- -CGGGGG-GaGGGG-CgUGUCUAC--------------CCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 22709 | 0.78 | 0.128843 |
Target: 5'- cGCCCgCCUCCCC-CGCGGggGGcGACGa -3' miRNA: 3'- -CGGGgGGAGGGGcGUGUCuaCC-CUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 22901 | 0.68 | 0.518635 |
Target: 5'- gGCCCgCCagccCCCCGCGgcCGGA-GGGACc -3' miRNA: 3'- -CGGGgGGa---GGGGCGU--GUCUaCCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 23258 | 0.72 | 0.29418 |
Target: 5'- cGCCgCCgCCUCCgCCGCgccGCGGGaGGGGCGc -3' miRNA: 3'- -CGG-GG-GGAGG-GGCG---UGUCUaCCCUGC- -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 23393 | 0.74 | 0.249618 |
Target: 5'- cGCCCCCCUCCCCGCGCccgcgccccccuccAcgcccccGGGGCc -3' miRNA: 3'- -CGGGGGGAGGGGCGUG--------------Ucua----CCCUGc -5' |
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5559 | 3' | -62.5 | NC_001806.1 | + | 23529 | 0.67 | 0.60459 |
Target: 5'- gGCCCCgaCC-CCCUGgGCGGcUGGcGGCGg -3' miRNA: 3'- -CGGGG--GGaGGGGCgUGUCuACC-CUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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